Application of plasma circulating microRNA-448, 506, 4316, and 4478 analysis for non-invasive diagnosis of lung cancer

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Tomasz PowrózekJanusz Milanowski

Abstract

Aberrant expression of microRNAs (miRNAs) in cancer patients compared to healthy people as well as possibility of detection of these molecules in blood samples make them potential biomarkers of various cancers. In the present study, we investigated the potential role of four miRNAs as lung cancer (LC) biomarkers: miRNA-448, 506, 4316, and 4478. Using quantitative reverse transcription polymerase chain reaction (qRT-PCR) technique, we assessed expression of studied miRNAs in plasma samples of 90 lung cancer patients and 85 healthy individuals. Receiver operating curves (ROC) with area under the curve (AUC) were used to assess accuracy of studied miRNAs for distinguishing LC patients from healthy individuals. The miRNA-448 and 4478 were significantly overexpressed in lung cancer patients compared to healthy people and these two molecules were qualified for further analysis. Combination ROC analysis of both biomarkers reached 90 % of sensitivity and 76.3 % of specificity (AUC = 0.896) for distinguishing operable (stage IA-IIB) non-small cell lung cancer (NSCLC) patients from healthy subjects. Our results suggest that the examination of miRNAs could be considered as potential lung cancer, non-invasive biomarkers.

References

Sep 23, 2006·Developmental Biology·Baohong ZhangTodd A Anderson
May 18, 2007·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Beth B McConnellVincent W Yang
Mar 1, 2008·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Giulia VeronesiLorenzo Spaggiari
Jul 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Patrick S MitchellMuneesh Tewari
Sep 26, 2008·The New England Journal of Medicine·Roy S HerbstScott M Lippman
Jan 25, 2011·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Kristen M FossGlen J Weiss
Jan 27, 2011·Pathology Oncology Research : POR·Kinga TóthZsolt Tulassay
Feb 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Mattia BoeriGabriella Sozzi
May 3, 2011·Current Genomics·Leigh-Ann Macfarlane, Paul R Murphy
May 23, 2012·JAMA : the Journal of the American Medical Association·Peter B BachFrank C Detterbeck
Sep 11, 2012·Cancer Research·Georgios NikolaidisTriantafillos Liloglou
Mar 7, 2013·European Journal of Cancer Prevention : the Official Journal of the European Cancer Prevention Organisation (ECP)·Dongfang TangYongjie Wang
Jan 15, 2014·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Gabriella SozziUgo Pastorino
Dec 19, 2014·Cancer Biomarkers : Section a of Disease Markers·Reza GhanbariSakari Knuutila
Apr 8, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jun DengJianping Xiong
Apr 16, 2015·BioMed Research International·Huiyin LanHongchuan Jin
Jun 17, 2015·Translational Research : the Journal of Laboratory and Clinical Medicine·Tomasz PowrózekJanusz Milanowski
Jun 25, 2015·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Y LvC Fang

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Citations

Apr 2, 2016·Medical Oncology·Chen ZhaoHonglei Chen
Jul 9, 2016·The Oncologist·Benjamin LevyDaniel Becker
Apr 28, 2017·Biomolecular Concepts·Virginie Armand-Labit, Anne Pradines
Mar 9, 2019·Molecular and Clinical Oncology·Laura GiuntiIacopo Sardi
Apr 14, 2017·International Journal of Colorectal Disease·Paweł KrawczykJanusz Milanowski
Aug 15, 2019·Experimental and Therapeutic Medicine·Hongfeng QiDabin Pang
Mar 1, 2020·Experimental and Molecular Pathology·Soudeh Ghafouri-FardMohammad Taheri
Mar 3, 2020·Life Sciences·Md Mahmodul Hasan Sohel
Dec 1, 2020·Translational Research : the Journal of Laboratory and Clinical Medicine·Shen ZhongJürgen Borlak
Apr 4, 2020·Genomics·Negin SoghliNegar Soghli
Aug 2, 2017·The Lancet Oncology·Jennifer BeaneAvrum Spira

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