Application of polymeric surfactants in micellar electrokinetic chromatography-electrospray ionization mass spectrometry of benzodiazepines and benzoxazocine chiral drugs

Electrophoresis
Jingguo HouS A Shamsi

Abstract

Chiral micellar EKC (CMEKC) coupled to ESI-MS using polymeric surfactants as pseudostationary phases is investigated for simultaneous enantioseparation of two benzodiazepines, (+/-)-oxazepam ((+/-)-OXA) and (+/-)-lorazepam ((+/-)-LOR), and one benzoxazocine, (+/-)-nefopam ((+/-)-NEF). First, enantioselectivity and electrospray sensitivity of six chiral polymeric surfactants for all three chiral compounds are compared. Second, using poly(sodium N-undecenoyl-L-leucinate) as pseudostationary phase, the organic modifiers (methanol (MeOH), isopropanol, and ACN) are added into the running buffer to further improve chiral resolution (RS). Next, a CMEKC-ESI-MS method for the simultaneous enantioseparation of two benzodiazepines is further developed by using a dipeptide polymeric surfactant, poly(sodium N-undecenoxy carbonyl-L,L-leucyl-valinate) (poly-L,L-SUCLV). The CMEKC conditions including nebulizer pressure, capillary length, ammonium acetate concentration, pH, poly-L,L-SUCLV concentration, and capillary temperature were optimized to achieve maximum chiral RS and highest sensitivity of MS detection. The spray chamber parameters (drying gas temperature and drying gas flow rate) as well as sheath liquid conditions (MeOH content, pH, ...Continue Reading

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Citations

May 20, 2008·Analytical Chemistry·Vratislav KostalEdgar A Arriaga
Feb 26, 2010·Journal of Chromatography. a·Govert W SomsenGerhardus J de Jong
Aug 19, 2008·Journal of Chromatography. a·Gerald Gübitz, Martin G Schmid
Aug 28, 2007·Journal of Chromatography. a·Christian NilssonStaffan Nilsson
Aug 5, 2009·Electrophoresis·Peter Mikus, Katarína Maráková
Mar 19, 2008·Journal of Separation Science·Christopher P Palmer
Jan 3, 2007·Journal of Mass Spectrometry : JMS
Dec 29, 2007·Electrophoresis·Laura Sánchez-HernándezCarmen García-Ruiz
Apr 9, 2010·Electrophoresis·Carolina SimóAlejandro Cifuentes
Apr 19, 2015·Journal of Pharmaceutical and Biomedical Analysis·Aneta WoźniakiewiczPaweł Kościelniak
Aug 17, 2020·Journal of Chromatography. a·Sandra Salido-FortunaMaría Luisa Marina

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