Application of poly(methacrylic acid-ethylene glycol dimethacrylate) monolith microextraction coupled with capillary zone electrophoresis to the determination of opiates in human urine

Electrophoresis
Fang WeiYu-Qi Feng

Abstract

A novel poly(methacrylic acid-ethylene glycol dimethacrylate) (MAA-EGDMA) monolith microextraction method coupled with CZE was proposed for rapidly determining a mixture of opiates comprising heroin, 6-monoacetylmorphine, morphine, codeine, papaverine, and narcotine in human urine. The extraction device contained a regular plastic syringe, the poly(MAA-EGDMA) monolithic capillary tube (530 microm id x 3 cm) and a plastic pinhead, which connected the monolithic capillary tube and the syringe without leakage. In the polymer monolith microextraction, the sample solution was ejected via the monolithic capillary tube by a programmable syringe pump, followed by desorption with an aliquot of appropriate solution, which was collected into a vial for the subsequent analysis by CZE. The best separation was achieved using a buffer composed of 0.1 M disodium hydrogen phosphate (adjusted to pH 4.5 with 1 M hydrochloric acid) and 20% methanol v/v with temperature and voltage of 25 degrees C and 25 kV, respectively. By applying electrokinetic injection with field-enhanced sample stacking, detection limits of 6.6-19.5 ng/mL were achieved. Excellent method of reproducibility was found over a linear range of 80-2000 ng/mL.

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Citations

Sep 21, 2010·Analytical and Bioanalytical Chemistry·Li XuYu-Qi Feng
Sep 12, 2012·Analytical and Bioanalytical Chemistry·Isabelle KohlerSerge Rudaz
Sep 9, 2011·Bioanalysis·Victoria SamanidouKonstantinos Rentifis
Aug 11, 2012·Bioanalysis·Mário BarrosoEugenia Gallardo
Sep 20, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Mojtaba Shamsipur, Nazir Fattahi
Jan 5, 2011·Journal of Pharmaceutical and Biomedical Analysis·Hiroyuki Kataoka, Keita Saito
Mar 3, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·M Lombardo-AgüíA M García-Campaña
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Oct 24, 2007·Peptides·Richard J Bodnar
Oct 31, 2009·Journal of Separation Science·María Asensio-RamosSalvatore Fanali
Jul 11, 2008·Journal of Separation Science·Oscar G Potter, Emily F Hilder
Dec 7, 2007·Electrophoresis·Franco Tagliaro, Federica Bortolotti
Dec 8, 2011·Electrophoresis·Heide R RabanesJoselito P Quirino
Mar 12, 2011·Journal of Separation Science·Akira NameraShota Miyazaki
Nov 21, 2009·Analytica Chimica Acta·Hiroyuki KataokaKeita Saito

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