Application of proteomics in biomarker discovery: a primer for the clinician.

Physiological Research
V TamborR Pudil

Abstract

Ever since proteomics was proven to be capable of characterizing a large number of differences in both protein quality and quantity, it has been applied in various areas of biomedicine, ranging from the deciphering molecular pathogenesis of diseases to the characterization of novel drug targets and the discovery of potential diagnostic biomarkers. Indeed, the biomarker discovery in human plasma is clearly one of the areas with enormous potential. However, without proper planning and implementation of specific techniques, the efforts and expectations may very easily be hampered. Numerous earlier projects aimed at clinical proteomics, characterized by exaggerated enthusiasm, often underestimated some principal obstacles of plasma biomarker discovery. Consequently, ambiguous and insignificant results soon led to a more critical view in this field. In this article, we critically review the current state of proteomic approaches for biomarker discovery and validation, in order to provide basic information and guidelines for both clinicians and researchers. These need to be closely considered prior to initiation of a project aimed at plasma biomarker discovery. We also present a short overview of recent applications of clinical proteo...Continue Reading

Citations

Nov 11, 2014·Journal of Proteome Research·Luis M TeranThomas Franz
Jun 7, 2011·Journal of Proteomics·Mutay Aslan, Serdar Dogan
Mar 23, 2011·Fetal Diagnosis and Therapy·Anna Lucia MastricciKypros H Nicolaides
Jun 30, 2012·Progress in Neurobiology·Simone ListaHarald Hampel
Sep 22, 2012·Proteomics. Clinical Applications·Ana Isabel PadrãoFrancisco Amado
Nov 9, 2012·Proteomics·Tzu-Chiao Chao, Nicole Hansmeier

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