Application of radially grown ZnO nanowires on poly-l-lactide microfibers complexed with a tumor antigen for cancer immunotherapy

Nanoscale
Prashant SharmaNam-Hyuk Cho

Abstract

Zinc oxide (ZnO)-based nanocomposites have shown promising potential for various biomedical applications, including vaccine development, owing to their multifunctionality and biocompatibility. Here, we synthesized radially grown ZnO nanowires (NWs) on poly-l-lactic acid (PLLA) microfibers with unique 3-dimensional structure and applied them as therapeutic cancer vaccines. This inorganic-organic hybrid nanocomposite has mild cellular toxicity but efficiently delivers a tumor antigen into dendritic cells, cellular bridges between innate and adaptive immunity, to stimulate them to express inflammatory cytokines and activation surface markers. We also demonstrated that the hybrid nanocomposites successfully induce tumor antigen-specific cellular immunity and significantly inhibit tumor growth in vivo. ZnO NWs on PLLA fibers systemically reduced immune suppressive TReg cells and enhanced the infiltration of T cells into tumor tissues, compared to mice immunized with PLLA fibers coated with the antigen. Our current findings open a new avenue in extending the biomedical application of inorganic metal oxide-inert organic hybrid nanocomposites as a novel vaccine platform.

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Citations

Jun 14, 2020·Journal of Functional Biomaterials·Giuseppe ArrabitoBruno Pignataro
Sep 29, 2019·Pharmaceutics·Prashant SharmaNam-Hyuk Cho
Jun 12, 2020·Biomimetics·Maria P Nikolova, Murthy S Chavali
Dec 18, 2020·Physical Review. E·Jing ZouXinlei Li
Jan 27, 2021·ACS Nano·Joshua SeabergPriyabrata Mukherjee
Apr 29, 2021·Macromolecular Bioscience·Ai Lin ChinRong Tong
May 6, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Zechun DangTongsheng Chen

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Methods Mentioned

BETA
scanning electron microscopy
X-ray
density gradient centrifugation
FACS
flow cytometry
electrophoresis

Software Mentioned

FlowJo

Related Concepts

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