Application of real-time polymerase chain reaction in detection of Entamoeba histolytica in pus aspirates of liver abscess patients

Foodborne Pathogens and Disease
Nurulhasanah OthmanRahmah Noordin

Abstract

Entamoeba histolytica is the second major cause of liver abscess disease in humans, particularly in developing countries. Recently, DNA molecular-based methods have been employed to enhance the detection of E. histolytica in either pus or stool specimens. In this study, the results of real-time polymerase chain reaction (PCR) to detect E. histolytica DNA in pus from liver abscess cases were compared with those of indirect hemagglutination assay on the corresponding serum samples. Bacterial cultures were also performed on the pus samples for the diagnosis of pyogenic liver abscess. The real-time PCR detected E. histolytica DNA in 23 of 30 (76.7%) pus samples, when compared with 14 of 30 (46.7%) serum samples in which anti-Entamoeba antibodies were detected by indirect hemagglutination assay and 4 of 30 (13.3%) pus samples that showed bacterial infection by culture. The use of real-time PCR is a promising detection method for diagnosis and epidemiology assessment of amoebic liver abscess.

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Citations

Jan 1, 2012·Asian Pacific Journal of Tropical Biomedicine·Tan Zi NingLim Boon Huat
Sep 16, 2011·Clinical and Vaccine Immunology : CVI·Weng Kin WongRahmah Noordin
May 18, 2013·Experimental Parasitology·Nurulhasanah OthmanRahmah Noordin
Mar 29, 2014·Experimental Biology and Medicine·Samson S Y WongKwok-Yung Yuen
Apr 12, 2016·Journal of Gastroenterology and Hepatology·Lucas H RijsmanJohannes G Kusters
Apr 4, 2014·Clinical Microbiology Reviews·Jaco J Verweij, C Rune Stensvold
Aug 5, 2010·Current Opinion in Infectious Diseases·Lisette van Lieshout, Jaco J Verweij
Aug 24, 2015·Clinical Liver Disease·Judith A Anesi, Stephen Gluckman
Jun 17, 2020·Pathogens and Global Health·Gaayathri KumarasamyNurulhasanah Othman
Sep 27, 2018·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Syazwan SaidinRahmah Noordin
Jul 1, 2019·Diagnosis·Albert Bernet SánchezMercè García González
Oct 1, 2020·The American Journal of Tropical Medicine and Hygiene·Rahmah NoordinRohela Mahmud

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Datasets Mentioned

BETA
AB282658

Methods Mentioned

BETA
ELISA
PCR

Software Mentioned

BLAST
BLAST search
SPSS

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