Application of resazurin for estimating abundance of contaminant-degrading micro-organisms

Letters in Applied Microbiology
T F GuerinB Peasley

Abstract

The aim of the current study was to test whether resazurin changed colour when incubated with a range of organic chemicals used as growth substrates in bioremediation studies and to determine whether resazurin was more effective in estimating microbial growth than turbidity alone (i.e. no resazurin) or use of the dye, methylene blue. Resazurin was incubated with a range of organic chemicals that were used as substrates in an MPN assay. Only 1,2-dichlorobenzene, 2,4-D, glycol sulphite and sulphinol reacted to generate false positives. Resazurin was also used to estimate micro-organisms in a series of bioremediation studies. The results showed that resazurin was more sensitive than methylene blue or turbidity alone as an indicator of microbial growth. The significance of the current study is that resazurin should be used in MPN assays for estimating contaminant-degrading micro-organisms instead of turbidity alone or other dyes such as methylene blue.

References

Sep 1, 1992·Bulletin of Environmental Contamination and Toxicology·P G Nix, M M Daykin
Jan 1, 1991·Archives of Microbiology·M A HeitkampC E Cerniglia
Jan 5, 2000·Applied and Environmental Microbiology·J F Alley, L R Brown
Feb 1, 1982·Applied and Environmental Microbiology·H KiyoharaK Yana
Mar 1, 1990·Applied and Environmental Microbiology·H G Song, R Bartha

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Citations

Mar 24, 2007·Applied Microbiology and Biotechnology·Seiki TakenoMasato Ikeda
Feb 10, 2009·Applied Microbiology and Biotechnology·Alberto MariscalJoaquin Fernandez-Crehuet
Jul 30, 2010·Journal of the Royal Society, Interface·Camelia E DijkstraRichard J A Hill
Jul 23, 2013·PloS One·Karina M WinnJames G Mitchell
May 12, 2009·Journal of Microbiological Methods·Malena E SandbergAdyary Fallarero
Mar 25, 2008·Journal of Hazardous Materials·Hyeoksun Choi, Joann Silverstein
Aug 4, 2015·Marine Pollution Bulletin·Turlough F Guerin
Oct 19, 2004·Journal of Microbiological Methods·Peter RoslevMartin Hesselsoe
Dec 30, 2014·Archives of Environmental Contamination and Toxicology·Renato Nallin MontagnolliEderio Dino Bidoia
Apr 22, 2016·ACS Applied Materials & Interfaces·Ott SchelerPiotr Garstecki
Sep 15, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Malena E SkogmanAdyary Fallarero
Nov 24, 2019·Journal of Applied Microbiology·R G LedderA J McBain
Oct 17, 2017·Biotechnology and Bioengineering·Jian Lin ChenDavid C Stuckey
Jul 28, 2019·Micromachines·Koji MatsuuraChao-Min Cheng
May 26, 2018·Science Advances·Alex SavchenkoElena Molokanova
Apr 3, 2020·Medical Mycology·Martin van EijkHans de Cock
Feb 21, 2006·Biosensors & Bioelectronics·Aynsley C TizzardGareth Lloyd-Jones
Jan 26, 2021·ACS Omega·Ruisheng Wang, David Erickson
Jan 17, 2020·Analytical Chemistry·Ruisheng WangDavid Erickson
Dec 8, 2020·ACS Applied Materials & Interfaces·Simona LencovaKamila Zdenkova

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