Application of (S)-N-(4-Nitrophenoxycarbonyl) phenylalanine methoxyethyl ester as a chiral derivatizing reagent for reversed-phase high-performance liquid chromatographic separation of diastereomers of amino alcohols, non-protein amino acids, and PenA

Amino Acids
Ravi Bhushan, Charu Agarwal

Abstract

Indirect enantioresolution of 15 primary and secondary amino group containing compounds (amino alcohols, non-protein amino acids, PenA) was done using the reagent (S)-N-(4-Nitrophenoxycarbonyl) phenylalanine methoxyethyl ester [(S)-NIFE] by reversed-phase high-performance liquid chromatography. The diastereomeric derivatives were analyzed under reversed-phase conditions using linear gradient. The detection was at 205 nm and sharp peaks were obtained. The reagent used is comparatively economic than the other derivatizing reagents. Method validation was also done.

References

Jul 2, 2003·Journal of Chromatography. a·Antal PéterFrans Borremans
Apr 3, 2004·Journal of Chromatography. a·Antal PéterDaniel W Armstrong
Nov 12, 2009·Biomedical Chromatography : BMC·Ravi Bhushan, Rajender Kumar

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Citations

Jul 28, 2012·Journal of Chromatography. a·István IliszAntal Péter
Apr 1, 2015·Journal of Chromatographic Science·Rituraj Dubey, Ravi Bhushan
Nov 3, 2004·Analytical Biochemistry·Boontarika BoonyapiwatGlyn B Steventon
Nov 16, 2021·Analytical and Bioanalytical Chemistry·Eliana A AgathokleousConstantina Kapnissi-Christodoulou

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