Application of serial analysis of gene expression to the study of the gene expression profile of Leishmania infantum chagasi promastigote.

Journal of Biomedicine & Biotechnology
Adelino Soares Lima NetoCarlos Henrique Nery Costa

Abstract

This study describes the application of the LongSAGE methodology to study the gene expression profile in promastigotes of Leishmania infantum chagasi. A tag library was created using the LongSAGE method and consisted of 14,208 tags of 17 bases. Of these, 8,427 (59.3%) were distinct. BLAST research of the 1,645 most abundant tags showed that 12.8% of them identified the coding sequences of genes, while 82% (1,349/1,645) identified one or more genomic sequences that did not correspond with open reading frames. Only 5.2% (84/1,645) of the tags were not aligned to any position in the L. infantum genome. The UTR size of Leishmania and the lack of CATG sites in some transcripts were decisive for the generation of tags in these regions. Additional analysis will allow a better understanding of the expression profile and discovering the key genes in this life cycle.

References

Oct 20, 1995·Science·V E VelculescuK W Kinzler
Jan 5, 2000·Proceedings of the National Academy of Sciences of the United States of America·J J ChenS M Wang
May 1, 2002·Nature Biotechnology·Saurabh SahaVictor E Velculescu
Mar 11, 2003·Nucleic Acids Research·Catheline VilainGilbert Vassart
Oct 18, 2003·Molecular and Biochemical Parasitology·Chaoqun YaoMary E Wilson
Nov 1, 2003·Sleep Medicine·Marco Zucconi
Nov 19, 2003·Microbes and Infection·David A CampbellNancy R Sturm
Dec 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Hideo MatsumuraRyohei Terauchi
Jul 1, 2004·Comparative Immunology, Microbiology and Infectious Diseases·P Desjeux
Dec 25, 2004·Cellular Microbiology·Hideo MatsumuraRyohei Terauchi
Jul 16, 2005·Science·Alasdair C IvensPeter J Myler
May 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Julius LukesMichael A Miles
Jun 19, 2007·Nature Genetics·Christopher S PeacockMatthew Berriman
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
May 20, 2009·Bioinformatics·Heng Li, Richard Durbin
Jul 27, 2010·International Journal for Parasitology·Pedro J AlcoleaVicente Larraga

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE29369

Methods Mentioned

BETA
PCR

Software Mentioned

RAGE
SAGE
Lasergene
SAGE2000
SuperSAGE
EditSeq
GLGI
LongSAGE
BLAST

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.