Application of shielded column liquid chromatography for determination of sulfamonomethoxine, sulfadimethoxine, and their N4-acetyl metabolites in milk

Journal of Chromatography. a
Kunihiro Kishida, N Furusawa


A hazardous-chemical free method for simultaneous determination of sulfamonomethoxine (SMM), sulfadimethoxine (SDM), and their N4-acetyl metabolites in raw milk using shielded column liquid chromatography is developed. The target analytes are extracted by mixing with ethanol-acetic acid (97:3, v/v) followed by centrifugation. The procedure uses a Hisep shielded hydrophobic phase (SHP) column, isocratic elution with 0.1% acetic acid solution (pH 3.1, in water)-ethanol (75:25, v/v), and a photo-diode array detector. Average recoveries from samples spiked at 25-500 ng/ml for each drug were >81% with relative standard deviations within 5%. The limits of quantitation were <25 ng/ml.


May 21, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xiao-Lin HouXiang-Dang Du
Apr 12, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Geun-Cheol GilGabriela S Chirica
Jul 16, 2013·Therapeutic Drug Monitoring·María E MarsonGuido Mastrantonio

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