Application of statistical design for the optimization of amino acid separation by reverse-phase HPLC

Analytical Biochemistry
R GheshlaghiP L Douglas

Abstract

Modified resolution and overall separation factors used to quantify the separation of complex chromatography systems are described. These factors were proven to be applicable to the optimization of amino acid resolution in reverse-phase (RP) HPLC chromatograms. To optimize precolumn derivatization with phenylisothiocyanate, a 2(5-1) fractional factorial design in triplicate was employed. The five independent variables for optimizing the overall separation factor were triethylamine content of the aqueous buffer, pH of the aqueous buffer, separation temperature, methanol/acetonitrile concentration ratio in the organic eluant, and mobile phase flow rate. Of these, triethylamine concentration and methanol/acetonitrile concentration ratio were the most important. The methodology captured the interaction between variables. Temperature appeared in the interaction terms; consequently, it was included in the hierarchic model. The preliminary model based on the factorial experiments was not able to explain the response curvature in the design space; therefore, a central composite design was used to provide a quadratic model. Constrained nonlinear programming was used for optimization purposes. The quadratic model predicted the optimal le...Continue Reading

References

Oct 1, 1988·Analytical Biochemistry·S A Cohen, D J Strydom
Aug 29, 1973·Journal of Chromatography·M Roth, A Hampaï
Jan 1, 1984·Analytical Biochemistry·S Stein, S Udenfriend
Jul 26, 2002·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·Mohamed A El-Sadek
Dec 4, 2002·Letters in Applied Microbiology·J RajendhranP Gunasekaran
Dec 31, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Lourdes BoschRosaura Farré

❮ Previous
Next ❯

Citations

May 21, 2011·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Anosheh RahmaniFarhang Soleimany
Mar 19, 2016·International Journal of Analytical Chemistry·Bürge AşçıÖzlem Aksu Dönmez
Jul 12, 2013·Pharmaceutical Biology·Mariana Guadalupe VallejoAlicia Mariel Agnese
May 3, 2016·Artificial Cells, Nanomedicine, and Biotechnology·Semra AkgönüllüAdil Denizli
May 19, 2017·Journal of Separation Science·Tamara Alvarez-SeguraM Celia García-Alvarez-Coque
Apr 25, 2020·Food Science & Nutrition·Samere DastangooSamira Yeganehzad
Dec 12, 2019·Frontiers in Plant Science·Xingxing FengXianzhong Feng
Jan 14, 2021·Environmental Science and Pollution Research International·Chunxia DingZhiyong Wen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.