Application of synthetic peptides for detection of anti-citrullinated peptide antibodies

Peptides
Nicole Hartwig TrierGunnar Houen

Abstract

Anti-citrullinated protein antibodies (ACPAs) are a hallmark of rheumatoid arthritis (RA) and represent an important tool for the serological diagnosis of RA. In this study, we describe ACPA reactivity to overlapping citrullinated Epstein-Barr virus nuclear antigen-1 (EBNA-1)-derived peptides and analyze their potential as substrates for ACPA detection by streptavidin capture enzyme-linked immunosorbent assay. Using systematically overlapping peptides, containing a 10 amino acid overlap, labelled with biotin C-terminally or N-terminally, sera from 160 individuals (RA sera (n=60), healthy controls (n=40), systemic lupus erythematosus (n=20), Sjögren's syndrome (n=40)) were screened for antibody reactivity. Antibodies to a panel of five citrullinated EBNA-1 peptides were found in 67% of RA sera, exclusively of the IgG isotype, while 53% of the patient sera reacted with a single peptide, ARGGSRERARGRGRG-Cit-GEKR, accounting for more than half of the ACPA reactivity alone. Moreover, these antibodies were detected in 10% of CCP2-negative RA sera. In addition, 47% of the RA sera reacted with two or three citrullinated EBNA-1 peptides from the selected peptide panel. Furthermore, a negative correlation between the biotin attachment si...Continue Reading

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Citations

Jun 28, 2016·Scandinavian Journal of Clinical and Laboratory Investigation·Catharina E DamNicole H Trier
Mar 8, 2017·Antibodies·Nicole H Trier, Gunnar Houen
Dec 19, 2019·International Journal of Molecular Sciences·Nicole TrierGunnar Houen
Sep 24, 2019·Antibodies·Nicole H TrierGunnar Houen

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