Application of the CellDetect® staining technique in diagnosis of human cervical cancer

Gynecologic Oncology
Song HeLi Chen

Abstract

CellDetect® staining technique is a newly invented technique for cancer diagnosis. It easily distinguishes between normal and neoplastic cells including pre-cancer and squamous cell carcinoma (SCC) cells, based on staining color and morphology. In this study, application of CellDetect® staining technique was assessed in diagnosis of human cervical cancer as compared with hematoxylin and eosin (H&E) staining in conventional slides and Thinprep cytologic test (TCT) smears. The conventional slides and TCT smears of 600 patients were stained and observed while comparing with H&E staining to assess sensitivity and specificity of CellDetect® staining technique in diagnosis of cervical cancer. Conventional smear slides (440 cases) were fixed in 95% ethanol or with CYTOFIX® Spray. TCT smears (160 cases) were processed based on manual. The paraffin sections from cervical intraepithelium neoplasia (CIN) 2-3 and SCC cases were prepared by biopsy. CellDetect® staining exhibited well cell morphology, simultaneously, showed dual color discrimination, the stain targeted cytoplasm in normal cells in green and dysplastic cells or neoplastic cells in purple/red. Both cervical cell smears or both fixation methods in conventional slides did not af...Continue Reading

References

Dec 22, 2009·Journal of Carcinogenesis·Idit SagivAlexander Levitzki
Mar 10, 2011·International Journal of Cancer. Journal International Du Cancer·Ju-Fang ShiYou-Lin Qiao
Jul 18, 2012·Asian Pacific Journal of Cancer Prevention : APJCP·Hua ChenBin-Lie Yang

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