PMID: 11906561Mar 22, 2002Paper

Application of the E-screen assay to test for oestrogenically active substances in swine feed

Journal of Animal Physiology and Animal Nutrition
N BitschH Brunn

Abstract

A pig breeder in central Hesse (Germany) noticed the occurrence of enlarged vulvae in female piglets. Intoxication with oestrogenically active substances by contamination of two feed mixes ingested by the mother sows appeared to be a possible cause. Using a combined technique of the DFG analytical method S19 and the E-screen assay, two feed samples were found to contain powerful oestrogenically active compounds. By co-incubation with the anti-oestrogen tamoxifen it could be clearly demonstrated that the oestrogenic activity was mediated by the oestrogen receptor. These results demonstrate that use of the E-screen assay in combination with the DFG analytical method S19 provides a simple and readily usable prescreening method for the routine detection of oestrogenically active compounds in animal feed. The results from the E-screen assay show that the sows ingested 10-80 microg oestradiol equivalents per day in their feed. Because of the bioavailability of these substances, the oestrogenic active compounds seem to be transferred into the milk and passed to the piglets via suckling. The milk of the dam appears to contain this substance in biologically active form and at such high concentrations that the female piglets had enlarged...Continue Reading

Citations

Sep 21, 2013·Bio Systems·Steven M CornsKenneth M Bryden
Jul 10, 2010·Zeitschrift für Kinder- und Jugendpsychiatrie und Psychotherapie·Jan FrölichGerd Lehmkuhl

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