PMID: 14423044Apr 1, 1960Paper

Application of the electron microscope to the cytochemical peroxidase reaction in salamander leukocytes

The Journal of Biophysical and Biochemical Cytology
T MITSUI

Abstract

The present study has dealt with the localization by electron microscopy of the products of peroxidase reaction in neutrophil leukocytes in the subcapsular region of the livers of Triturus viridescens. Small pieces of liver tissue were fixed for 1 hour in buffered osmium tetroxide solution. After fixation they were divided into five groups: (a) Not treated with any reagent (control); (b) Treated for 4 minutes with the peroxidase reagent containing 0.3 per cent benzidine and 0.014 per cent (0.004 molar) hydrogen peroxide in 50 per cent alcohol; (c) Treated for 4 minutes with 0.3 per cent benzidine solution in 50 per cent alcohol alone (control); (d) Treated for 4 minutes with 0.014 per cent (0.004 molar) hydrogen peroxide in 50 per cent alcohol alone (control); (e) Treated for 5 minutes with pure methanol, washed in water, and treated for 4 minutes with the peroxidase reagent (inhibition test). Each group was then dehydrated and embedded in either methacrylate or epoxy resin. In electron micrographs, the reaction products of peroxidase activity were evidenced in the form of dense materials localized in the specific granules in the cytoplasm of the neutrophil leukocytes. Neither mitochondria nor any other particles showed increas...Continue Reading

References

Nov 1, 1952·The Anatomical Record·G E PALADE
Jan 1, 1953·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R D LILLIE, H J BURTNER
Jul 1, 1953·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·G E PALADE
Dec 1, 1955·Experimental Cell Research·H SHELDOND BRANDES
Nov 25, 1955·The Journal of Biophysical and Biochemical Cytology·G E PALADE
Jul 25, 1956·The Journal of Biophysical and Biochemical Cytology·H SWIFT
Jul 25, 1957·The Journal of Biophysical and Biochemical Cytology·R J BARRNETT, G E PALADE
Jan 25, 1958·The Journal of Biophysical and Biochemical Cytology·J S TRIER
Mar 25, 1958·The Journal of Biophysical and Biochemical Cytology·A M GLAUERT, R H GLAUERT
Jan 25, 1959·The Journal of Biophysical and Biochemical Cytology·R L WOOD, C C HOWARD
Jan 1, 1955·The Journal of Biophysical and Biochemical Cytology·G E PALADE

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Citations

Feb 27, 1969·The New England Journal of Medicine·S J Klebanoff, L R White
Jan 1, 1970·The Journal of Cell Biology·W G Forssmann, L Girardier
Apr 1, 1971·Experimental Parasitology·S Akao

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