PMID: 9546409Apr 18, 1998Paper

Application of the enzyme-linked immunoelectrotransfer blot to filter paper blood spots to estimate seroprevalence of cysticercosis in Bolivia

The American Journal of Tropical Medicine and Hygiene
H S JafriV C Tsang

Abstract

An enzyme-linked immunoelectrotransfer blot (EITB) assay was used to study the prevalence of cysticercosis in rural Bolivia. Dried blood spots on filter paper from fingersticks were used as assay samples. Before the serosurvey, experiments were performed to show that samples eluted from dried whole blood on filter paper exhibited no decrease in sensitivity when compared with the more traditional serum samples used in the EITB. Fingerstick blood dried on filter paper is a convenient, economical way of transporting and storing field samples for epidemiologic surveys of cysticercosis in developing countries. This report shows the utility of this sample collection method in underdeveloped countries where refrigeration is not possible and where venipuncture is a problem. Blood was obtained from randomly selected residents in three rural regions of Bolivia: Chuquisaca (n = 1,859), Cochabamba (n = 1,516), and Tarija (n = 1,010). The estimated seroprevalence on 10% of the sample collected for the three regions were 9%, 4.5%, and 2%, respectively.

Citations

Feb 1, 2003·Haemophilia : the Official Journal of the World Federation of Hemophilia·R WinikoffG-E Rivard
Mar 2, 2012·Emerging Infectious Diseases·Seth E O'NealWilliam M Stauffer
Dec 23, 2016·African Journal of Laboratory Medicine·Sam L NsobyaBarbara S Mensch
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Mar 17, 2006·Molecular and Biochemical Parasitology·Kathy HancockVictor C W Tsang

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