PMID: 20650128Aug 1, 1995Paper

application of the human dermal model skin(2) ZK 1350 to phototoxicity and skin corrosivity testing

Toxicology in Vitro : an International Journal Published in Association with BIBRA
M LiebschHorst Spielmann

Abstract

The human three-dimensional in vitro model Skin(2) ZK 1350(TM) [Advanced Tissue Sciences (ATS), La Jolla, USA] was tested for two in vitro toxicology applications, prediction of phototoxicity and classification of skin corrosivity. For phototoxicity testing chemicals were applied topically for 1 or 24 hr followed by 30 min of irradiation with a non-irritating dose of UVA. Phototoxicity was assessed 24 hr later by comparing cytotoxicity of UVA-exposed and non-exposed tissue in the MTT assay. In a European EC/COLIPA in vitro phototoxicity validation trial with 20 test chemicals (11 phototoxic and nine non-phototoxic), the best result was obtained with the Skin(2) ZK 1350 assay in the 24-hr exposure protocol where nine of the 11 phototoxins were classified correctly both at ATS and ZEBET. 6-Methylcoumarin could only be identified as a phototoxin when applied through the medium to the dermis side of the skin model for 24 hr. All of the nine non-phototoxic chemicals were identified correctly with either 1- or 24-hr preincubation. To classify chemicals as corrosive to the skin with an in vitro assay, ZEBET and two other laboratories tested 50 chemicals under blind conditions in the Skin(2) ZK 1350 model within a European ECVAM valida...Continue Reading

References

Aug 1, 1994·Toxicology in Vitro : an International Journal Published in Association with BIBRA·H SpielmannA Willshaw
Jan 1, 1991·Toxicology in Vitro : an International Journal Published in Association with BIBRA·R Osborne, M A Perkins

❮ Previous
Next ❯

Citations

Jul 10, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·P A JonesR S Lawrence
Jun 1, 1998·Toxicology in Vitro : an International Journal Published in Association with BIBRA·H SpielmannP Brantom
Aug 1, 1998·Toxicology in Vitro : an International Journal Published in Association with BIBRA·J H FentemM Liebsch
Mar 7, 2001·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·V AndrisanoV Cavrini
Jan 7, 2004·Mutation Research·Susanne Brendler-SchwaabDietmar Utesch
May 28, 2008·International Journal of Cosmetic Science·R RoguetM H Teissier
Dec 17, 2014·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·A M ApiD K Wilcox
Oct 13, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Nathalie DijouxPierre-Jacques Ferret
Jun 29, 2005·International Journal of Toxicology·Amy R Elmore, UNKNOWN Cosmetic Ingredient Review Expert Panel
Mar 27, 2018·Experimental Dermatology·Christian RiebelingTewes Tralau
Jan 29, 2010·Alternatives to Laboratory Animals : ATLA·John SheasgreenDavid Ingalls
Oct 1, 2005·Alternatives to Laboratory Animals : ATLA·Manfred LiebschChantra Eskes
Dec 4, 2016·International Journal of Toxicology·Wilbur JohnsonF Alan Andersen
Dec 6, 2000·Alternatives to Laboratory Animals : ATLA·H SpielmannUNKNOWN European Centre for Validation of Alternative Methods
May 1, 2000·Alternatives to Laboratory Animals : ATLA·M LiebschH G Holzhütter
Mar 16, 2021·Biogerontology·Sophia Letsiou
Jul 3, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Damien LelièvreJosé Cotovio
Jul 30, 2019·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·A M ApiS Tsang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.