PMID: 9170168Feb 1, 1997Paper

Application of the new stereological probes to the study of the melanosome in Cloudman S91 melanoma cells

Pigment Cell Research
D FangB Weatherhead

Abstract

The relationship between melanosome size and number and melanin content has been investigated in Cloudman S91 melanoma cells growing in vitro using both "model-based" and "design-based" stereological procedures. Cells were cultured for 4 days, harvested at daily intervals, and resin-embedded for light and electron microscopy; one aliquot of each sample of cells was assayed to determine its melanin content. By comparing their volume-weighted mean nuclear volume and their number-weighted mean nuclear volume, we have found that the nuclei of Cloudman melanoma cells form a fairly homogeneous population. The volume fraction and absolute volume of premelanosomes (VVpm, cell and Vpm) and mature melanosomes (VVmm, cell and Vmm) were all found to decrease progressively throughout the period of culture as did the number of premelanosomes (Npm) and mature melanosomes (Nmm). Whilst the volume-weighted mean volume of individual stage I and stage II premelanosomes, (VVipm), remained fairly constant at about 10 nm3, the volume of individual stage III and IV mature melanosomes showed significant variation ranging between about 13 nm3 and 32 nm3. The melanin content of the cells decreased progressively over the 4 days of culture. There were, ho...Continue Reading

References

Jun 1, 1992·Pathology, Research and Practice·A MaioranaS Garbisa
Nov 1, 1973·Analytical Biochemistry·M H RosenthalR Shiman
Jun 1, 1993·Pigment Cell Research·W S OettingR A King

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Citations

Jul 27, 2001·The International Journal of Biochemistry & Cell Biology·M Martínez-EsparzaA Zuasti
Sep 18, 2009·Experimental Dermatology·Søren KampTomas Norman Dam
Aug 5, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mikhail O DurymanovAlexander S Sobolev

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