Application of two-dimensional gel electrophoresis to interrogate alterations in the proteome of genetically modified crops. 2. Assessing natural variability

Journal of Agricultural and Food Chemistry
Martin C RuebeltKlaus-Dieter Jany

Abstract

Proteomics is currently tested as a complementary tool for the safety assessment of genetically modified (GM) crops. Understanding the natural variability of the proteome is crucial for the interpretation of biological differences between transgenic and nontransgenic parental lines. The natural variation of seed protein profiles among a set of 12 Arabidopsis thaliana ecotypes was determined by utilizing two-dimensional electrophoresis (2DE). The total number of different resolved protein spots found among the 12 ecotypes was 931 with a range of 573 (Mt-0) to 653 (Condara) in any one ecotype. Although the ecotypes were grown side-by-side in an environmentally controlled growth chamber, almost half of the resolved spots varied with respect to their presence/absence, and 95% of the spots present in all ecotypes varied in spot quantity (2-53-fold). In the evaluation of unintended effects of genetic modification, it is concluded that the experimental design must account for existing natural variability, which, in the case of the expressed proteome, can be substantial.

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Citations

Nov 30, 2006·Journal of Applied Genetics·Marcin Filipecki, Stefan Malepszy
Nov 15, 2008·Functional & Integrative Genomics·Christine FinnieBirte Svensson
Oct 1, 2011·Journal of Agricultural and Food Chemistry·Geisi M BalsamoAna C M Arisi
Aug 10, 2010·Critical Reviews in Food Science and Nutrition·Romina PedreschiBart Nicolaï
Jun 1, 2011·Environment International·Jack A HeinemannDavid Quist
Jun 1, 2010·Regulatory Toxicology and Pharmacology : RTP·Reiko TeshimaRyosuke Nakamura
Mar 2, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Rika NakamuraReiko Teshima
Jul 27, 2007·Proteomics·Jesús V JorrínMa Angeles Castillejo
Sep 26, 2007·Proteomics·Natasha A Karp, Kathryn S Lilley
Feb 2, 2010·Electrophoresis·Gabriella Stefania ScippaAndrea Scaloni
Jun 3, 2015·Journal of Proteome Research·Subhra ChakrabortySetsuko Komatsu
Jul 1, 2017·Journal of Agricultural and Food Chemistry·Gianluca ParediAndrea Mozzarelli
Apr 19, 2011·Mass Spectrometry Reviews·Virginia García-CañasAlejandro Cifuentes
Mar 5, 2011·Mass Spectrometry Reviews·Miguel HerreroAlejandro Cifuentes
Sep 5, 2018·Scientific Reports·Michael N AntoniouGilles-Eric Séralini
Dec 23, 2008·Journal of Proteome Research·Mariasole Di CarliAngiola Desiderio
Oct 26, 2010·Journal of Proteome Research·Mikko J AnttonenSirpa O Kärenlampi

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