PMID: 20654119Jul 27, 2010Paper

Application of Warthin-Starry stain, immunohistochemistry and transmission electron microscopy in diagnosis of cat scratch disease

Zhonghua bing li xue za zhi Chinese journal of pathology
Juan HuangFang-lei Duan

Abstract

To evaluate the diagnostic utility of Warthin-Starry silver stain, immunohistochemistry and transmission electron microscopy in the detection of human Bartonella henselae infection and pathologic diagnosis of cat scratch disease (CSD). The paraffin-embedded lymph node tissues of 77 histologically-defined cases of cat scratch disease collected during the period from January, 1998 to December, 2008 were retrieved and studied using Warthin-Starry silver stain (WS stain) and mouse monoclonal antibody against Bartonella henselae (BhmAB stain). Five cases rich in bacteria were selected for transmission electron microscopy. Under electron microscope, the organisms Bartonella henselae appeared polymorphic, round, elliptical, short rod or bacilliform shapes, ranged from 0.489 to 1.110 microm by 0.333 to 0.534 microm and often clustered together. Black short rod-shaped bacilli arranged in chains or clumps were demonstrated in 61.0% (47/77) of CSD by WS stain. The organisms were located outside the cells and lie mainly in the necrotic debris, especially near the nodal capsule. In 72.7% (56/77) of the cases, dot-like, granular as well as few linear positive signals were observed using BhmAB immunostain and showed similar localization. Posi...Continue Reading

Related Concepts

Related Feeds

Cat-scratch disease

Cat-scratch disease, a relatively common infectious disease, is caused by Bartonella henselae and characterized by a fever and regional lymphadenitis. Here is the latest research.

Bartonellosis

Bartonellosis is an infection caused by bacteria in the genus Bartonella and can present as a variety of diseases depending on the organism involved. While asymptomatic infection has been reported, immunocompromised individuals are more susceptible to severe infection. Find the latest research on bartonellosis here.

Related Papers

The Canadian Journal of Infectious Diseases = Journal Canadien Des Maladies Infectieuses
M A Opavsky
Revue de stomatologie et de chirurgie maxillo-faciale
L Ben SlamaB Royer
© 2021 Meta ULC. All rights reserved