PMID: 16538031Mar 16, 2006Paper

Application study of intracytoplasmic sperm injection for golden hamster and cattle production

The Journal of Reproduction and Development
Toshitaka Horiuchi

Abstract

This paper describes several technical improvements and our results in hamster intracytoplasmic sperm injection (ICSI), hamster round spermatid injection (ROSI) and bovine ICSI. The hamster is the mammalian species in which ICSI was first tried to produce fertilized oocytes. However, until recently, no live offspring following ICSI have ever been obtained. We reported the birth of live offspring following hamster ICSI. Improved points to success were 1) performing hamster ICSI in a dark room with a small incandescent lamp and manipulating both oocytes and fertilized eggs under microscope with a red light source and 2) injecting sperm heads without acrosomes. Under controlled illumination, the majority of the oocytes injected with acrosomeless sperm heads were fertilized normally, cleaved, and developed into morulae. Nine live offspring (19%) were born by transfer of hamster ICSI-derived embryos. Furthermore, we reported the birth of live offspring following hamster ROSI. About 70% of oocytes injected with round spermatids broken before injection were fertilized normally and about half of them developed to morulae and blastocysts. Three (5%) live young were born by transfer of hamster ROSI-derived embryos. On the other hand, in ...Continue Reading

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Citations

Oct 25, 2008·Theriogenology·J XuF Du
Jun 19, 2007·Theriogenology·Raffaele BoniElisabetta Tosti
Feb 13, 2021·Genes·Veena UnnikrishnanJacob Thundathil

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