Applications of alginate in bioseparation of proteins

Artificial Cells, Blood Substitutes, and Immobilization Biotechnology
Sulakshana JainMunishwar N Gupta

Abstract

Alginate is a polysaccharide that is a block polymer consisting of block units of guluronic acid and mannuronic acid. It shows inherent biological affinity for a variety of enzymes such as pectinase, lipase, phospholipase D, a and ss amylases and glucoamylase. Taking advantage of its precipitation with Ca2+ and the above-mentioned property, alginate has been used for purification of these enzymes by affinity precipitation, aqueous two phase separation, macroaffinity ligand facilitated three phase partitioning, immobilized metal affinity chromatography and expanded bed affinity chromatography. Thus, this versatile marine resource has tremendous potential in bioseparation of proteins.

References

Mar 1, 1990·Trends in Biotechnology·O Smidsrød, G Skjåk-Braek
Feb 13, 1987·Journal of Chromatography·G Johansson, G Kopperschläger
Apr 1, 1994·Journal of Biomedical Materials Research·N M PopeA K Smith
Jun 1, 1995·The Journal of Applied Bacteriology·I SafaríkS J Forsythe
Aug 1, 1994·Trends in Biotechnology·H A Chase
Jul 1, 1997·The Journal of Pharmacy and Pharmacology·F A JohnsonA D Mercer
Jan 29, 2000·Protein Expression and Purification·A SharmaM N Gupta
Feb 16, 2000·Journal of Chromatography. a·F B AnspachW D Deckwer
Apr 25, 2001·Applied Biochemistry and Biotechnology·S Teotia, M N Gupta
Jun 8, 2001·Biotechnology and Applied Biochemistry·S Sharma, M N Gupta
Aug 21, 2001·Journal of Chromatography. a·S Teotia, M N Gupta
Dec 18, 2001·Analytical Biochemistry·Ipsita Roy, Munishwar N Gupta
Dec 18, 2001·Journal of Molecular Recognition : JMR·S TeotiaM N Gupta
Jan 19, 2002·Annals of the New York Academy of Sciences·D Poncelet
Apr 9, 2002·Molecular Biotechnology·Sunita Teotia, M N Gupta
May 7, 2002·Journal of Chromatography. a·Ipsita Roy, Munishwar N Gupta
Jun 8, 2002·International Journal of Pharmaceutics·B Y ChoiJ B Park
Jun 18, 2002·Artificial Cells, Blood Substitutes, and Immobilization Biotechnology·Marián NavrátilDaniela Smogrovicová
Sep 5, 2002·Biotechnology and Bioengineering·Aparna Sharma, M N Gupta
Mar 26, 2003·Protein Expression and Purification·Kalyani MondalMunishwar Nath Gupta
Apr 18, 2003·Biomaterials·Eugene Khor, Lee Yong Lim
Jun 13, 2003·Journal of Chromatography. a·Aparna SharmaMunishwar Nath Gupta
Oct 29, 2003·Journal of Biotechnology·M SafaríkováI Safarík
Dec 19, 2003·Protein Expression and Purification·Kalyani MondalMunishwar N Gupta
Jan 1, 2004·Chemistry & Biology·Ipsita Roy, Munishwar Nath Gupta
Feb 7, 2004·Journal of Chromatography. a·S Teotia, M N Gupta
Mar 23, 2004·Biotechnology and Applied Biochemistry·Canh Le-TienMircea-Alexandru Mateescu
May 25, 2004·Biotechnology and Applied Biochemistry·Sulakshana Jain, Munishwar N Gupta
Sep 11, 2004·Current Opinion in Biotechnology·Karl-Erich Jaeger, Thorsten Eggert
Oct 7, 2004·Current Opinion in Biotechnology·Todd M PrzybycienLandon M Steele
Oct 7, 2004·Current Opinion in Biotechnology·Alois JungbauerRobert Schlegl
Oct 13, 2004·Protein Expression and Purification·Seon-Kap HwangThomas W Okita
Nov 1, 1987·Biotechnology and Bioengineering·F TjerneldM Joelsson

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Citations

Aug 12, 2014·Drug Development and Industrial Pharmacy·Dharmendra Jain, Daniel Bar-Shalom
Feb 5, 2010·Journal of Bioscience and Bioengineering·Ekambaram NakkeeranSukumaran Umesh-Kumar
Jan 9, 2008·Journal of Biomedical Materials Research. Part a·Helen H LuRegina Landesberg
Apr 21, 2006·Biochimica Et Biophysica Acta·Kalyani MondalMunishwar N Gupta
Jan 21, 2021·Polymers·Ronny G Huamani-PalominoAna C Valderrama

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