Applications of CometChip for Environmental Health Studies.

Chemical Research in Toxicology
Christy Chao, Bevin P Engelward

Abstract

Environmental exposures have long been known to impact public health and safety. For example, exposures to airborne particulates, heavy metals in water, or certain industrial chemicals can contribute to aging and to risk of developing cancer and other diseases. Environmental factors can impact health in a variety of ways, but a key concern is DNA damage, which can lead to mutations that cause cancer. Cancer can take years to develop following chemical exposure; however, one way to predict carcinogenicity in a more practical time frame is by studying the chemical's ability to induce DNA damage. The comet assay (or single-cell gel electrophoresis assay) has been used successfully for genotoxicity testing. The comet assay allows for the detection of DNA strand breaks via analysis of DNA migration during electrophoresis. Previously, the Engelward laboratory, in collaboration with the Bhatia laboratory, developed the CometChip for measurements of DNA damage and repair. The CometChip is a high-throughput comet assay that improves user reproducibility and significantly shortens total assay time. Here, we describe how the high-throughput CometChip platform can be used to measure DNA damage in established cell lines, animal models, and ...Continue Reading

References

Feb 1, 1992·Experimental Cell Research·P L OliveJ P Banáth
Mar 1, 1988·Experimental Cell Research·N P SinghE L Schneider
Aug 30, 1984·Biochemical and Biophysical Research Communications·O Ostling, K J Johanson
Feb 1, 1995·Mutation Research·D W FairbairnK L O'Neill
Jul 1, 1993·Trends in Genetics : TIG·A P Grollman, M Moriya
Jan 1, 1996·Environmental and Molecular Mutagenesis·S Pfuhler, H U Wolf
Apr 16, 1998·The Journal of Biological Chemistry·E P RogakouW M Bonner
Mar 29, 2000·Environmental and Molecular Mutagenesis·R R TiceY F Sasaki
Aug 6, 2002·Free Radical Biology & Medicine·Jean CadetJean-Luc Ravanat
Mar 3, 2004·Archives of Biochemistry and Biophysics·Peter C Dedon, Steven R Tannenbaum
Jan 8, 2005·Chembiochem : a European Journal of Chemical Biology·Orlando D Schärer
May 19, 2005·Mutagenesis·Susanne Brendler-SchwaabGünter Speit
Jun 21, 2005·Toxicology and Applied Pharmacology·Weiling Xue, David Warshawsky
Apr 5, 2007·Nature Protocols·Peggy L Olive, Judit P Banáth
Nov 28, 2007·Cell Biology and Toxicology·T S KumaravelAwadhesh N Jha
Feb 7, 2008·Mutagenesis·Gillian R WassonC Stephen Downes
Feb 8, 2008·Mutagenesis·Declan J McKennaValerie J McKelvey-Martin
Mar 11, 2008·Mutagenesis·Maria Dusinska, Andrew R Collins
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·David K WoodBevin P Engelward
Jun 28, 2011·Nature Reviews. Cancer·Andrew J Deans, Stephen C West
Nov 2, 2011·DNA Repair·James T MutambaBevin P Engelward
Jan 13, 2012·Nature Reviews. Cancer·Dragony FuLeona D Samson
Jul 27, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Ming-Wei ChaoGerald N Wogan
Mar 7, 2013·Mutagenesis·Kristine B GutzkowGunnar Brunborg
Apr 27, 2013·Biochimica Et Biophysica Acta·Andrew R Collins
May 8, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Jing GeBevin P Engelward
Jun 24, 2014·Environmental Science. Nano·Georgios A SotiriouPhilip Demokritou
Sep 10, 2014·Frontiers in Genetics·Amaya AzquetaAndrew Collins
Oct 29, 2014·Journal of Visualized Experiments : JoVE·Jing GeBevin P Engelward
Nov 13, 2014·Frontiers in Genetics·Gunnar BrunborgKristine B Gutzkow
Mar 17, 2015·Environmental and Molecular Mutagenesis·Kai RothkammSusanne Burdak-Rothkamm

❮ Previous
Next ❯

Citations

Nov 19, 2020·Trends in Cancer·Norah A OwitiBevin P Engelward

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.