Applications of Deep Mutational Scanning in Virology.

Viruses
Thomas D Burton, Nicholas S Eyre

Abstract

Several recently developed high-throughput techniques have changed the field of molecular virology. For example, proteomics studies reveal complete interactomes of a viral protein, genome-wide CRISPR knockout and activation screens probe the importance of every single human gene in aiding or fighting a virus, and ChIP-seq experiments reveal genome-wide epigenetic changes in response to infection. Deep mutational scanning is a relatively novel form of protein science which allows the in-depth functional analysis of every nucleotide within a viral gene or genome, revealing regions of importance, flexibility, and mutational potential. In this review, we discuss the application of this technique to RNA viruses including members of the Flaviviridae family, Influenza A Virus and Severe Acute Respiratory Syndrome Coronavirus 2. We also briefly discuss the reverse genetics systems which allow for analysis of viral replication cycles, next-generation sequencing technologies and the bioinformatics tools that facilitate this research.

References

Sep 15, 1996·Nucleic Acids Research·J ClineH H Hogrefe
May 14, 2003·Journal of Virology·Sang-Im YunYoung-Min Lee
Oct 22, 2004·Emerging Infectious Diseases·Min-Shi Lee, Jack Si-En Chen
Feb 25, 2005·FEMS Microbiology Letters·Pramila Walpita, Ramon Flick
Mar 16, 2005·Analytical Biochemistry·Thomas VanherckePeter Denolf
Sep 29, 2006·Bioinformatics·Mario A Fares, David McNally
Apr 21, 2007·Nature Protocols·Manfred T Reetz, José Daniel Carballeira
Apr 16, 2008·Annual Review of Biochemistry·Rhiju Das, David Baker
Nov 7, 2008·Nature·David R BentleyAnthony J Smith
Nov 22, 2008·Science·John EidStephen Turner
Feb 21, 2009·Vaccine·Nicole M Bouvier, Peter Palese
Feb 21, 2009·Vaccine·M F DucatezR J Webby
Apr 8, 2009·Nature Nanotechnology·James ClarkeHagan Bayley
May 9, 2009·The New England Journal of Medicine·Fatimah S DawoodTimothy M Uyeki
Dec 22, 2009·Future Microbiology·Benjamin G HaleAdolfo García-Sastre
Jan 19, 2010·Nature Methods·Joseph B HiattJay Shendure
Jun 12, 2010·The Journal of Biological Chemistry·Stéphane BoivinDarren J Hart
Jul 2, 2010·Journal of Virology·Natalia A IlyushinaRichard J Webby
May 28, 2011·Immunity·Yueh-Ming Loo, Michael Gale
Feb 9, 2012·Mobile DNA·Brian GreenBrendan P Cormack
Feb 14, 2012·Structure·Bharat V AdkarRaghavan Varadarajan
Jun 21, 2012·Influenza and Other Respiratory Viruses·Othmar G Engelhardt
Jul 10, 2012·Biochimica Et Biophysica Acta·Henrique NemésioJosé Villalaín
Aug 24, 2012·Journal of Virology·Lucy ThorneIan Goodfellow
Nov 22, 2012·Viruses·Carolien E van de SandtGuus F Rimmelzwaan
Mar 14, 2013·Nature Communications·Lauren TurrellFrank T Vreede
Apr 9, 2013·Nature·Samir BhattSimon I Hay
Apr 16, 2013·Antiviral Research·Nick S Laursen, Ian A Wilson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Landau-Kleffner Syndrome

Landau Kleffner syndrome (LKS), also called infantile acquired aphasia, acquired epileptic aphasia, or aphasia with convulsive disorder, is a rare childhood neurological syndrome characterized by the sudden or gradual development of aphasia (the inability to understand or express language) and an abnormal electroencephalogram. Discover the latest research on LKS here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Related Papers

Frontiers in Molecular Biosciences
Krishna K Narayanan, Erik Procko
Bulletin of the New York State Society of Dentistry for Children
J H Kauffmann
Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Miguel E Quiñones-MateuMiguel A Martinez
Science
Vineet N KewalRamani, John M Coffin
© 2021 Meta ULC. All rights reserved