Applications of flow cytometry in environmental microbiology and biotechnology

Extremophiles : Life Under Extreme Conditions
Peter L BergquistTristrom Winsley

Abstract

Flow cytometry (FCM) is a technique for counting, examining and sorting microscopic particles suspended in a stream of fluid. It uses the principles of light scattering, light excitation and the emission from fluorescent molecules to generate specific multiparameter data from particles and cells. The cells are hydrodynamically focussed in a sheath solution before being intercepted by a focused light source provided by a laser. FCM has been used primarily in medical applications but is being used increasingly for the examination of individual cells from environmental samples. It has found uses in the isolation of both culturable and hitherto non-culturable bacteria present infrequently in environmental samples using appropriate growth conditions. FCM lends itself to high-throughput applications in directed evolution for the analysis of single cells or cell populations carrying mutant genes. It is also suitable for encapsulation studies where individual bacteria are compartmentalised with substrate in water-in-oil-in-water emulsions or with individual genes in transcriptional/translational mixtures for the production of mutant enzymes. The sensitivity of the technique has allowed the examination of gene optimisation by a procedur...Continue Reading

References

Jul 1, 1993·The Journal of Applied Bacteriology·Graham VeseyC R Fricker
Nov 15, 1997·Applied and Environmental Microbiology·G WallnerR Amann
May 9, 1998·Applied and Environmental Microbiology·P J BellDuncan Veal
Jul 14, 1998·Nature Biotechnology·Dan S Tawfik, Andrew D Griffiths
Aug 10, 1999·Journal of Bacteriology·T J Beveridge
Sep 15, 2000·Journal of Immunological Methods·Duncan VealP V Attfield
Jan 3, 2001·Applied and Environmental Microbiology·Lars Hestbjerg HansenSøren J Sørensen
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·Farid J GhadessyPhilipp Holliger
Sep 1, 2001·Biochimie·C BretonC Jeanneau
Nov 20, 2002·Proceedings of the National Academy of Sciences of the United States of America·Karsten ZenglerMartin Keller
Dec 31, 2002·The EMBO Journal·Andrew D Griffiths, Dan S Tawfik
Jun 20, 2003·Methods in Cell Science : an Official Journal of the Society for in Vitro Biology·Hazel M Davey
Sep 23, 2003·Protein Science : a Publication of the Protein Society·Cornelius BesslerRolf D Schmid
Oct 24, 2003·Trends in Biotechnology·Nicholas J Turner
Mar 3, 2004·Protein Engineering, Design & Selection : PEDS·Farid J Ghadessy, Philipp Holliger
Mar 26, 2004·Nature Reviews. Microbiology·Martin Keller, Karsten Zengler
Mar 30, 2004·Antimicrobial Agents and Chemotherapy·Martin Iain BahlSøren J Sørensen
Jul 13, 2004·Nucleic Acids Research·Nobuhide DoiHiroshi Yanagawa
Feb 19, 2005·Current Opinion in Structural Biology·Cintia RoodveldtDan S Tawfik
Apr 7, 2005·Current Opinion in Chemical Biology·Amir AharoniDan S Tawfik
Nov 2, 2005·Methods in Enzymology·Karsten ZenglerMartin Keller
Dec 22, 2005·Nature Biotechnology·Jean-Denis PédelacqGeoffrey S Waldo
Mar 23, 2006·Microbial Cell Factories·Diethard Mattanovich, Nicole Borth
Apr 4, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jesse D BloomFrances H Arnold
May 17, 2006·Current Opinion in Biotechnology·Brian D Green, Martin Keller
Jun 23, 2006·Nature Methods·Oliver J MillerAndrew D Griffiths
Jul 18, 2006·Trends in Biotechnology·Andrew D Griffiths, Dan S Tawfik
Jul 25, 2006·Nature Methods·Amir AharoniStephen G Withers
Aug 29, 2006·Journal of Bioscience and Bioengineering·Tomoaki Matsuura, Tetsuya Yomo
Oct 24, 2006·Trends in Biotechnology·Achim RotheBarbara E Power
Jan 18, 2007·Chembiochem : a European Journal of Chemical Biology·Valerie TalyAndrew D Griffiths
Feb 7, 2007·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Belinda Ferrari, Peter L Bergquist
May 4, 2007·Chemical Communications : Chem Comm·Bernard T KellyAndrew D Griffiths
Feb 15, 2008·Journal of Biological Engineering·Marianne F KramerDaniel V Lim
Feb 21, 2008·Current Opinion in Chemical Biology·Shimon Bershtein, Dan S Tawfik
May 23, 2008·Journal of Molecular Biology·Shimon BershteinDan S Tawfik
Jun 14, 2008·Current Opinion in Microbiology·Thomas IshoeyRoger S Lasken
Jun 20, 2008·Current Opinion in Microbiology·Kamila CzechowskaJan Roelof van der Meer
Jun 25, 2008·Annual Review of Biophysics·Christian JäckelDonald Hilvert
Aug 21, 2008·Nature Protocols·Belinda FerrariSvend Binnerup
Sep 27, 2008·Nature·Philip Hugenholtz, Gene W Tyson
Oct 22, 2008·Nature Methods·Rinkoo Devi Gupta, Dan S Tawfik
Dec 5, 2008·Microbiology and Molecular Biology Reviews : MMBR·Victor KuninPhilip Hugenholtz
Mar 24, 2009·Applied and Environmental Microbiology·Belinda Ferrari, M R Gillings
Mar 31, 2009·International Journal of Molecular Sciences·Raquel Ibáñez-PeralBelinda Ferrari

Citations

Mar 31, 2010·Analytical and Bioanalytical Chemistry·Frederik Hammes, Thomas Egli
Mar 24, 2010·Applied Microbiology and Biotechnology·Satoshi IshiiKeishi Senoo
Feb 20, 2013·Marine Biotechnology·Sumihiro KoyamaTaishi Tsubouchi
Oct 17, 2009·Applied Biochemistry and Biotechnology·Elizabeth HardimanPeter L Bergquist
Dec 1, 2011·Nature Methods·Fedor V SubachVladislav V Verkhusha
Jan 13, 2010·Expert Opinion on Drug Metabolism & Toxicology·Santosh Kumar
Dec 25, 2010·Microbes and Infection·Dennis J HorvathSheryl S Justice
Oct 18, 2015·Gene·Rimantas Kodzius, Takashi Gojobori
Dec 18, 2013·Environmental Microbiology·Pablo Iván NikelVíctor de Lorenzo
May 5, 2011·Biotechnology Advances·R Lencastre FernandesK V Gernaey
Mar 31, 2015·Environmental Science and Pollution Research International·Tarik AbzazouRosa M Araujo
Jan 10, 2013·FEMS Microbiology Reviews·Paul C Blainey
Apr 13, 2019·Annual Review of Analytical Chemistry·Hong LianXiaomei Yan
Jan 24, 2017·Frontiers in Cellular and Infection Microbiology·Jacques Y B KhalilBernard La Scola
Dec 30, 2018·Water Research·Hannah R Safford, Heather N Bischel

Related Concepts

Biotechnology
Flow Cytometry
Mutagenesis Process
Body Fluids
Environment
Genes
Transcription, Genetic
Laboratory Culture
Medical Examination
Particle

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Related Papers

Current Opinion in Microbiology
Kamila CzechowskaJan Roelof van der Meer
Microbiology and Molecular Biology Reviews : MMBR
Byron F Brehm-Stecher, Eric A Johnson
Cytometry. Part a : the Journal of the International Society for Analytical Cytology
Howard M Shapiro
© 2021 Meta ULC. All rights reserved