Applications of fluorescence microscopy to studies of dental hard tissue

Frontiers of Medical and Biological Engineering : the International Journal of the Japan Society of Medical Electronics and Biological Engineering
H MatsumotoT Araki

Abstract

In this review we describe applications of fluorescence microscopy to investigations of dental hard tissue. Many studies have shown that human teeth show autofluorescence and several applications of fluorescence microscopy have been reported, although the chemical nature of autofluorescence remains unknown. However, most applications are based on a static measurement mode, which has inherent limitations. These limitations can be overcome by the use of time-resolved fluorescence microscopy. Therefore, we attempted to combine ordinary and time-resolved fluorescence microscopy to study human dentine. Using stationary measurements, blue fluorescence with a peak of 440 +/- 10 nm and a width of approximately 100 nm was observed. Increases in fluorescence intensity were found to be dependent upon age and temperature, regardless of tooth type or gender. The results suggest that the aging mechanism for crown dentine differs from that for root dentine and that the observed increase in fluorescence intensity is partially due to a temperature-dependent physicochemical mechanism. We speculate that fluorescence microscopy will become a useful tool for analysis of dental hard tissue in several fields.

Citations

Jun 19, 2014·Dental Materials : Official Publication of the Academy of Dental Materials·Shuai LuJi-hua Chen
Oct 14, 2004·Applied Spectroscopy·Eric J Bukowski, Frank V Bright
Oct 31, 2018·Journal of Esthetic and Restorative Dentistry : Official Publication of the American Academy of Esthetic Dentistry ... [et Al.]·Claudia Angela Maziero VolpatoFilipe Samuel Silva
Jun 9, 2021·Journal of Esthetic and Restorative Dentistry : Official Publication of the American Academy of Esthetic Dentistry ... [et Al.]·Faezeh AtriEdward A McLaren
Jun 29, 2021·Stomatologii︠a︡·E E DyakonenkoI Yu Lebedenko

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