Applications of High-Throughput Clonogenic Survival Assays in High-LET Particle Microbeams

Frontiers in Oncology
Antonios GeorgantzoglouR Jena

Abstract

Charged particle therapy is increasingly becoming a valuable tool in cancer treatment, mainly due to the favorable interaction of particle radiation with matter. Its application is still limited due, in part, to lack of data regarding the radiosensitivity of certain cell lines to this radiation type, especially to high-linear energy transfer (LET) particles. From the earliest days of radiation biology, the clonogenic survival assay has been used to provide radiation response data. This method produces reliable data but it is not optimized for high-throughput microbeam studies with high-LET radiation where high levels of cell killing lead to a very low probability of maintaining cells' clonogenic potential. A new method, therefore, is proposed in this paper, which could potentially allow these experiments to be conducted in a high-throughput fashion. Cells are seeded in special polypropylene dishes and bright-field illumination provides cell visualization. Digital images are obtained and cell detection is applied based on corner detection, generating individual cell targets as x-y points. These points in the dish are then irradiated individually by a micron field size high-LET microbeam. Post-irradiation, time-lapse imaging foll...Continue Reading

References

Feb 1, 1997·Radiation and Environmental Biophysics·M ScholzG Kraft
Jun 4, 1998·Clinical Oncology : a Journal of the Royal College of Radiologists·S C Short, M C Joiner
Feb 15, 2001·International Journal of Radiation Oncology, Biology, Physics·M C JoinerI Turesson
Nov 2, 2001·Clinical & Experimental Metastasis·T DemuthA Perneczky
Jun 6, 2003·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·U AgeroO N Mesquita
Sep 5, 2003·Cancer Biotherapy & Radiopharmaceuticals·John C RoeskeJacob Rotmensch
May 1, 1956·The Journal of Experimental Medicine·T T PUCK, P I MARCUS
Mar 4, 2005·Physics in Medicine and Biology·Michael B AltmanJohn C Roeske
May 20, 2005·Methods in Molecular Medicine·Anupama MunshiRaymond E Meyn
Jul 5, 2005·The Lancet Oncology·Kevin M PriseKathryn D Held
Jan 27, 2006·Radiation Research·Markus HeissGisela Taucher-Scholz
Feb 23, 2007·Radiation Protection Dosimetry·O VeselovJ Styczen
Apr 5, 2007·Nature Protocols·Nicolaas A P FrankenChris van Bree
Jul 7, 2007·Methods in Cell Biology·John C Crocker, Brenton D Hoffman
Jan 12, 2008·Journal of Radiation Research·Munetoshi MaedaKatsumi Kobayashi
Aug 8, 2008·Applied Radiation and Isotopes : Including Data, Instrumentation and Methods for Use in Agriculture, Industry and Medicine·G FlaccaventoB Vojnovic
Dec 2, 2009·Nature Reviews. Clinical Oncology·Marco Durante, Jay S Loeffler
Nov 30, 2010·Radiation Protection Dosimetry·K M Prise, G Schettino
Jan 17, 2012·International Journal of Radiation Oncology, Biology, Physics·Maarten NiemantsverdrietRobert P Coppes
Feb 15, 2012·Physics in Medicine and Biology·Alejandro CarabeHarald Paganetti
Mar 30, 2012·Physics in Medicine and Biology·Ph BarberetH Seznec
Sep 12, 2012·Scientific Reports·C FournierS Ritter
Sep 29, 2012·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Kazuhiko OonishiTadashi Kamada
Jan 5, 2013·Journal of Radiation Research·Teruaki KonishiYoshiyuki Shirakawa
Apr 2, 2013·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Hélène RiquierCarine Michiels
Jul 16, 2013·Clinical Oncology : a Journal of the Royal College of Radiologists·M E LomaxP O'Neill
Aug 22, 2013·Radiation and Environmental Biophysics·J C G JeynesK J Kirkby
Aug 4, 2015·Physics in Medicine and Biology·A GeorgantzoglouR Jena

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
X-ray
transfection
irradiate
irradiating
Fluorescence microscopy
fluorescence imaging

Software Mentioned

MATLAB

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.