Applications of next generation sequencing in molecular ecology of non-model organisms

Heredity
Robert Ekblom, J Galindo

Abstract

As most biologists are probably aware, technological advances in molecular biology during the last few years have opened up possibilities to rapidly generate large-scale sequencing data from non-model organisms at a reasonable cost. In an era when virtually any study organism can 'go genomic', it is worthwhile to review how this may impact molecular ecology. The first studies to put the next generation sequencing (NGS) to the test in ecologically well-characterized species without previous genome information were published in 2007 and the beginning of 2008. Since then several studies have followed in their footsteps, and a large number are undoubtedly under way. This review focuses on how NGS has been, and can be, applied to ecological, population genetic and conservation genetic studies of non-model species, in which there is no (or very limited) genomic resources. Our aim is to draw attention to the various possibilities that are opening up using the new technologies, but we also highlight some of the pitfalls and drawbacks with these methods. We will try to provide a snapshot of the current state of the art for this rapidly advancing and expanding field of research and give some likely directions for future developments.

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Citations

Mar 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·George H PerryWebb Miller
Aug 27, 2013·Genome Biology and Evolution·Owen G OsborneDmitry A Filatov
Feb 10, 2012·Molecular Biology and Evolution·Tovah SalcedoDebashish Bhattacharya
Nov 13, 2012·Applied and Environmental Microbiology·Sophie Sanchez-FerandinFabien Joux
Mar 16, 2012·BMC Genomics·Nicole GruenheitPeter Lockhart
Aug 10, 2013·BMC Genomics·Alberto PallaviciniGiuseppe Scapigliati
Jun 26, 2013·Genome Biology·Elizabeth L ClareLars Chittka
May 11, 2012·PloS One·Mohamed SalemJianbo Yao
Feb 26, 2013·PloS One·Julia T VilstrupLudovic Orlando
Jul 23, 2014·PloS One·Marcela Uliano-SilvaMauro de Freitas Rebelo
Dec 20, 2013·International Journal of Molecular Sciences·Ming Rui LiLin Feng Li

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