Applications of on-line weak affinity interactions in free solution capillary electrophoresis

Electrophoresis
N H HeegaardDavid D Y Chen

Abstract

The impressive selectivity offered by capillary electrophoresis can in some cases be further increased when ligands or additives that engage in weak affinity interactions with one or more of the separated analytes are added to the electrophoresis buffer. This on-line affinity capillary electrophoresis approach is feasible when the migration of complexed molecules is different from the migration of free molecules and when separation conditions are nondenaturing. In this review, we focus on applying weak interactions as tools to enhance the separation of closely related molecules, e.g., drug enantiomers and on using capillary electrophoresis to characterize such interactions quantitatively. We describe the equations for binding isotherms, illustrate how selectivity can be manipulated by varying the additive concentrations, and show how the methods may be used to estimate binding constants. On-line affinity capillary electrophoresis methods are especially valuable for enantiomeric separations and for functional characterization of the contents of biological samples that are only available in minute quantities.

References

Sep 11, 1992·Journal of Chromatography·J C KraakH Poppe
Jul 24, 1992·Journal of Medicinal Chemistry·Y H ChuG M Whitesides
Sep 15, 1991·Analytical Chemistry·A Guttman, N Cooke
Jan 1, 1991·Methods in Enzymology·W A LimA D Lander
May 1, 1991·Journal of Biochemical and Biophysical Methods·H KajiwaraK Oono
Oct 7, 1994·Journal of Chromatography. a·N H Heegaard
Aug 1, 1996·European Journal of Biochemistry·N H HeegaardF A Robey
Jan 1, 1996·Analytical Chemistry·L B KoutnyM Fuchs
Feb 10, 1998·Electrophoresis·G RippelJ Frank
Feb 10, 1998·Electrophoresis·K L Rundlett, D W Armstrong
Aug 26, 1998·Cellular and Molecular Life Sciences : CMLS·Y H Chu, C C Cheng
Sep 23, 1998·Chemico-biological Interactions·P K Sikka, K E McMartin
Oct 29, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·N H HeegaardN A Guzman
Oct 29, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·J Kawaoka, F A Gomez
Nov 21, 1998·Analytical Chemistry·I GermanR T Kennedy

❮ Previous
Next ❯

Citations

Mar 8, 2008·Electrophoresis·Maojun GongWilliam R Heineman
Jan 25, 2007·Electrophoresis·Sergey N Krylov
Jun 12, 2009·Electrophoresis·Niels H H Heegaard
Dec 15, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Norberto A Guzman, Daniel E Guzman
Jul 1, 2017·Electrophoresis·Vojtech Adam, Marketa Vaculovicova

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.