Applications of polymer brushes in protein analysis and purification

Annual Review of Analytical Chemistry
Parul JainMerlin L Bruening

Abstract

This review examines the application of polymer brush-modified flat surfaces, membranes, and beads for protein immobilization and isolation. Modification of porous substrates with brushes yields membranes that selectively bind tagged proteins to give 99% pure protein at capacities as high as 100 mg of protein per cubic centimeter of membrane. Moreover, enrichment of phosphopeptides on brush-modified matrix-assisted laser desorption/ionization (MALDI) plates allows detection and characterization of femtomole levels of phosphopeptides by MALDI mass spectrometry. Because swollen hydrophilic brushes can resist nonspecific protein adsorption while immobilizing a high density of proteins, they are attractive as substrates for protein microarrays. This review highlights the advantages of polymer brush-modified surfaces over self-assembled monolayers and identifies some research needs in this area.

References

Nov 6, 2001·Journal of Biochemical and Biophysical Methods·H ZouD Zhou
Dec 26, 2001·Chemical Reviews·K Matyjaszewski, J Xia
Jan 18, 2003·Analytical Biochemistry·Paul PelusoSteffen Nock
Mar 11, 2003·Proteomics·Wlad KusnezowJörg D Hoheisel
Mar 14, 2003·Nature·Eric PhizickyStanley Fields
Apr 5, 2003·Biotechnology Progress·Satoshi KobayashiWilliam Lee
Apr 12, 2003·Biotechnology and Applied Biochemistry·Heather N EndresMark R Etzel
May 28, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tomomi KawaiWilliam Lee
Dec 31, 2003·Analytical Chemistry·Isabelle CaelenLennart Wahlström
Jan 23, 2004·Chemical Society Reviews·Steve EdmondsonWilhelm T S Huck
Mar 18, 2004·Journal of the American Chemical Society·Chenjie XuBing Xu
May 12, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·M Yakup AricaGülay Bayramoğlu
May 21, 2004·Nature·Shunai CheTakashi Tatsumi
Mar 9, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Luisa AndruzziChristopher K Ober
Mar 23, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Juergen GrollMartin Moeller
May 10, 2005·Biomacromolecules·Stefano TuguluHarm-Anton Klok
Apr 19, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Jinhua DaiMerlin L Bruening
May 17, 2006·Journal of Molecular Recognition : JMR·Irina N SavinaBo Mattiasson
Jul 13, 2006·Journal of the American Chemical Society·Zhiyi BaoGregory L Baker
May 16, 2007·Journal of the American Chemical Society·Cyrille BoyerChristopher Barner-Kowollik
Feb 22, 1991·Science·S T Milner
Sep 21, 2007·Biomacromolecules·Rong DongChristopher K Ober
Oct 9, 2007·Biotechnology Progress·Akio IwanadeTakanobu Sugo
Oct 9, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Parul JainMerlin L Bruening
Nov 21, 2007·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Cuifang CaiThomas Kissel
Dec 7, 2007·Colloids and Surfaces. B, Biointerfaces·Ryoko IwataKazunari Akiyoshi
Dec 14, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Sean P CullenPadma Gopalan
Apr 9, 2008·Biotechnology and Bioengineering·Karl F Schilke, Christine Kelly
May 30, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Merlin L BrueningGregory L Baker
Jun 27, 2008·Analytical Chemistry·Jamie D DunnMerlin L Bruening
Jul 17, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Yasuhiko IwasakiRyoko Iwata
Mar 6, 2009·Mass Spectrometry Reviews·Jamie D DunnMerlin L Bruening

❮ Previous
Next ❯

Citations

Apr 4, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Somnath BhattacharjeeMerlin L Bruening
Jan 15, 2014·Omics : a Journal of Integrative Biology·Firas H KobeissyKevin Wang
Jan 13, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Xiaoying ZhuG Julius Vancso
Mar 31, 2016·Analytica Chimica Acta·María Vergara-BarberánJosé Manuel Herrero-Martínez
Jan 27, 2015·Biosensors & Bioelectronics·Mashooq Khan, Soo-Young Park
Jan 5, 2011·Acta Biomaterialia·Qian YuHong Chen
May 24, 2015·Annual Review of Analytical Chemistry·Jinlan Dong, Merlin L Bruening
May 28, 2016·The Journal of Physical Chemistry. B·Guang ChenSiddhartha Das
Oct 7, 2016·ACS Applied Materials & Interfaces·John J KeatingGeorges Belfort
Apr 8, 2017·Journal of Separation Science·Alexis G HuckabeeMary J Wirth
Jun 14, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Ekram WasselMohammed Es-Souni
May 2, 2020·Chemical Communications : Chem Comm·Yasuhiko IwasakiVoravee P Hoven
Feb 10, 2011·Macromolecular Bioscience·Kristopher D BarbeeXiaohua Huang
Sep 25, 2015·Soft Matter·Siddhartha DasRabibrata Mukherjee
Jun 14, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Mashooq KhanJin-Ming Lin
Sep 25, 2017·The Journal of Chemical Physics·Vinicius B Tergolina, Alexandre P Dos Santos
Nov 12, 2019·Analytical and Bioanalytical Chemistry·Ignacio PedreGraciela Alicia González
Oct 22, 2017·Journal of Biomedical Materials Research. Part a·Volker EtteltMichael Veith
Jun 25, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Benjamin S FlavelJ Justin Gooding
Apr 14, 2018·Chemistry of Materials : a Publication of the American Chemical Society·Xunzhi LiAlexander A Shestopalov
Aug 1, 2013·The Journal of Physical Chemistry. B·M Nuruzzaman KhanMichael Zharnikov
Nov 16, 2011·The Journal of Physical Chemistry. B·Cunfu ZhangJiang Zhao

❮ Previous
Next ❯

Methods Mentioned

BETA
size-exclusion chromatography
affinity purification
Fluorescence

Related Concepts

Related Feeds

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.