Applications of pressure perturbation calorimetry to study factors contributing to the volume changes upon protein unfolding

Biochimica Et Biophysica Acta
Pranav P Pandharipande, George I Makhatadze

Abstract

Pressure perturbation calorimetry (PPC) is a biophysical method that allows direct determination of the volume changes upon conformational transitions in macromolecules. This review provides novel details of the use of PPC to analyze unfolding transitions in proteins. The emphasis is made on the data analysis as well as on the validation of different structural factors that define the volume changes upon unfolding. Four case studies are presented that show the application of these concepts to various protein systems. The major conclusions are: 1. Knowledge of the thermodynamic parameters for heat induced unfolding facilitates the analysis of the PPC profiles. 2. The changes in the thermal expansion coefficient upon unfolding appear to be temperature dependent.3.Substitutions on the protein surface have negligible effects on the volume changes upon protein unfolding. 4. Structural plasticity of proteins defines the position dependent effect of amino acid substitutions of the residues buried in the native state. 5. Small proteins have positive volume changes upon unfolding which suggests difference in balance between the cavity/void volume in the native state and the hydration volume changes upon unfolding as compared to the larg...Continue Reading

References

Jan 1, 1977·Annual Review of Biophysics and Bioengineering·F M Richards
Aug 7, 1990·Biochemistry·K A Dill
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R L Baldwin
Dec 1, 1993·Protein Science : a Publication of the Protein Society·G I MakhatadzeP L Privalov
Jan 1, 1995·Advances in Protein Chemistry·G I Makhatadze, P L Privalov
Jan 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C N PaceK Gajiwala
Mar 6, 1999·Current Opinion in Microbiology·K Horikoshi
May 18, 1999·Journal of Molecular Biology·G DesaiC A Royer
Jun 23, 2001·Protein Science : a Publication of the Protein Society·V V LoladzeG I Makhatadze
Jul 21, 2001·Biophysical Journal·J Liang, K A Dill
May 2, 2002·Biochimica Et Biophysica Acta·Catherine A Royer
Jun 25, 2002·Journal of Molecular Biology·Vakhtang V LoladzeGeorge I Makhatadze
Jan 1, 1959·Advances in Protein Chemistry·W KAUZMANN
Feb 20, 2004·Journal of the American Chemical Society·Joseph D BatchelorGary J Pielak
May 14, 2004·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Revanur Ravindra, Roland Winter
Dec 24, 2005·Proteins·Devin G BarrettGary J Pielak
Jan 5, 2006·Journal of the American Chemical Society·Werner W Streicher, George I Makhatadze
Jan 18, 2007·Extremophiles : Life Under Extreme Conditions·Federico M Lauro, Douglas H Bartlett
Oct 24, 2007·Methods in Molecular Biology·P D Heiko Heerklotz
Oct 17, 2008·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Christina KrywkaRoland Winter
Feb 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Alexey V GribenkoGeorge I Makhatadze
Oct 20, 2009·Journal of Molecular Biology·Tigran V Chalikian, Robert B Macgregor
Nov 3, 2009·Biochemistry·Katrina L SchweikerGeorge I Makhatadze
Mar 30, 2010·Biophysical Chemistry·Soyoung LeeTigran V Chalikian
Jun 2, 2010·Biophysical Journal·Jean-Baptiste RougetCatherine A Royer
Sep 14, 2010·The Journal of Physical Chemistry. B·Alekos D TsamaloukasGeorge I Makhatadze
Jan 25, 2011·Biophysical Chemistry·Yong ZhaiRoland Winter
Jan 1, 2009·Methods in Enzymology·Katrina L Schweiker, George I Makhatadze
Nov 28, 2012·Science·Ken A Dill, Justin L MacCallum
May 23, 2014·The Journal of Physical Chemistry. B·Daniel VasilchukGeorge I Makhatadze
Jan 21, 2015·Methods : a Companion to Methods in Enzymology·Pranav P Pandharipande, George I Makhatadze
Apr 18, 2015·BMC Bioinformatics·Calvin R Chen, George I Makhatadze
Jul 16, 2015·Sub-cellular Biochemistry·Kunihiko Gekko

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Citations

Oct 25, 2016·Biochemistry·Heinrich KrobathHue Sun Chan
Feb 9, 2017·Nature Communications·Calvin R Chen, George I Makhatadze
Mar 20, 2019·Biophysics Reviews·Ryota WakayamaDamien Hall

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