Applications of the CRISPR/Cas9 System for Rice Grain Quality Improvement: Perspectives and Opportunities

International Journal of Molecular Sciences
Sajid FiazFahad Ali

Abstract

Grain quality improvement is a key target for rice breeders, along with yield. It is a multigenic trait that is simultaneously influenced by many factors. Over the past few decades, breeding for semi-dwarf cultivars and hybrids has significantly contributed to the attainment of high yield demands but reduced grain quality, which thus needs the attention of researchers. The availability of rice genome sequences has facilitated gene discovery, targeted mutagenesis, and revealed functional aspects of rice grain quality attributes. Some success has been achieved through the application of molecular markers to understand the genetic mechanisms for better rice grain quality; however, researchers have opted for novel strategies. Genomic alteration employing genome editing technologies (GETs) like clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) for reverse genetics has opened new avenues of research in the life sciences, including for rice grain quality improvement. Currently, CRISPR/Cas9 technology is widely used by researchers for genome editing to achieve the desired biological objectives, because of its simple targeting. Over the past few years many genes that are related to var...Continue Reading

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Citations

Jun 11, 2020·International Journal of Molecular Sciences·Waquar A AnsariRupesh Deshmukh
Jan 10, 2020·Briefings in Functional Genomics·Shakeel AhmadShaoqing Tang
Jul 24, 2020·International Journal of Molecular Sciences·Baohong Zhang
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Apr 28, 2021·Journal of the Science of Food and Agriculture·Min XiXueyuan Sun
Jun 3, 2021·International Journal of Molecular Sciences·Sajid FiazKi-Hung Jung
Jun 3, 2021·International Journal of Molecular Sciences·Tosin Victor AdegokeJian Zhang
Jun 29, 2021·Frontiers in Plant Science·Young-Cheon KimSeonghoe Jang
Jul 30, 2021·Frontiers in Plant Science·Ke ChenMariusz Jaremko
Aug 31, 2021·Frontiers in Plant Science·Yu DingTao Zhang
Sep 1, 2021·Plant Biotechnology Journal·Suozhen HuiPeisong Hu

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Methods Mentioned

BETA
gene knockout
transgenic
targeted mutations

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