Applied biocatalysis beyond just buffers - from aqueous to unconventional media. Options and guidelines.

Green Chemistry : an International Journal and Green Chemistry Resource : GC
Morten M C H van SchieDörte Rother

Abstract

In nature, enzymes conventionally operate under aqueous conditions. Because of this, aqueous buffers are often the choice for reaction media when enzymes are applied in chemical synthesis. However, to meet the demands of an industrial application, due to the poor water solubility of many industrially relevant compounds, an aqueous reaction system will often not be able to provide sufficient substrate loadings. A switch to a non-aqueous solvent system can provide a solution, which is already common for lipases, but more challenging for biocatalysts from other enzyme classes. The choices in solvent types and systems, however, can be overwhelming. Furthermore, some engineering of the protein structure of biocatalyst formulation is required. In this review, a guide for those working with biocatalysts, who look for a way to increase their reaction productivity, is presented. Examples reported clearly show that bulk water is not necessarily required for biocatalytic reactions and that clever solvent systems design can support increased product concentrations thereby decreasing waste formation. Additionally, under these conditions, enzymes can also be combined in cascades with other, water-sensitive, chemical catalysts. Finally, we sh...Continue Reading

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Citations

May 15, 2021·Chemical Communications : Chem Comm·Friederike E H NintzelFrank Hollmann
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Pablo Domínguez de María
Sep 25, 2021·Chembiochem : a European Journal of Chemical Biology·Martin PengKersten S Rabe
Oct 13, 2021·Chemical Communications : Chem Comm·José ColomaUlf Hanefeld

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