Applying genomics to the avian inner ear: development of subtractive cDNA resources for exploring sensory function and hair cell regeneration

Genomics
R David HawkinsM Lovett

Abstract

We applied a micro-cDNA-based subtraction method to identify genes expressed in the regenerating sensory epithelia (SE) of the chicken inner ear. Sensory hair cells in the avian utricle SE are in a constant state of turnover, where dying hair cells are replaced by new ones derived from supporting cells. In contrast, hair cells in the cochlea remain quiescent unless damaged. We used this difference to enrich for utricle-specific genes, using reiterative cDNA subtraction and demonstrate enrichment for utricle-specific sequences. A total of 1710 cDNA sequence reads revealed the presence of many cDNAs encoding known structural components of the SE (for example, Harmonin and beta-tectorin), proteins involved in cellular proliferation, such as P311, HIPK2, and SPALT1, among many others of unknown function. These libraries are the first of their kind and should prove useful for the discovery of candidate genes for hearing disorders, regenerative and apoptotic pathways, and novel chicken ESTs.

References

Jun 24, 1988·Science·J T Corwin, D A Cotanche
Oct 1, 1987·Archives of Otolaryngology--head & Neck Surgery·R M CruzE W Rubel
May 1, 1993·The Journal of Comparative Neurology·P Weisleder, E W Rubel
Apr 1, 1993·Hearing Research·E C Oesterle, E W Rubel
Sep 1, 1996·Genome Research·M F BonaldoM B Soares
Jun 1, 1999·Methods in Enzymology·A D Simmons, M Lovett
Sep 14, 2000·Developmental Biology·E R Farrell, A E Münsterberg
Jan 19, 2002·Biochemical and Biophysical Research Communications·Giovanna Maria PierantoniAlfredo Fusco
Apr 5, 2002·Genome Research·W James Kent
Jun 4, 2002·The Journal of Biological Chemistry·Naoya FujitaTakashi Tsuruo
May 2, 2003·Nature·Mireille MontcouquiolMatthew W Kelley
Feb 26, 2004·Audiology & Neuro-otology·Mark A Parker, Douglas A Cotanche
Aug 21, 2004·Development·Kate F Barald, Matthew W Kelley
Sep 11, 2004·Human Molecular Genetics·R David Hawkins, Michael Lovett
Oct 16, 2004·Journal of Neurochemistry·Masashi FujitaniToshihide Yamashita
Oct 20, 2004·The Journal of Cell Biology·Amanda K WigginsEric J Huang
Dec 14, 2004·Nature·UNKNOWN International Chicken Genome Sequencing Consortium
Apr 15, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Alexander DaviesJulian Lewis

❮ Previous
Next ❯

Citations

Apr 24, 2012·PloS One·Kara E PowderMichael Lovett
Oct 30, 2018·Frontiers in Pharmacology·Leslie StradiotLeo A van Grunsven

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Auditory Perception

Auditory perception is the ability to receive and interpret information attained by the ears. Here is the latest research on factors and underlying mechanisms that influence auditory perception.