Applying metabolomic analyses to the practice of embryology: physiology, development and assisted reproductive technology

Reproduction, Fertility, and Development
Rebecca L KrisherWilliam B Schoolcraft

Abstract

The advent of metabolomics technology and its application to small samples has allowed us to non-invasively monitor the metabolic activity of embryos in a complex culture environment. The aim of this study was to apply metabolomics technology to the analysis of individual embryos from several species during in vitro development to gain an insight into the metabolomics pathways used by embryos and their relationship with embryo quality. Alanine is produced by both in vivo- and in vitro-derived human, murine, bovine and porcine embryos. Glutamine is also produced by the embryos of these four species, but only those produced in vitro. Across species, blastocysts significantly consumed amino acids from the culture medium, whereas glucose was not significantly taken up. There are significant differences in the metabolic profile of in vivo- compared with in vitro-produced embryos at the blastocyst stage. For example, in vitro-produced murine embryos consume arginine, asparagine, glutamate and proline, whereas in vivo-produced embryos do not. Human embryos produce more alanine, glutamate and glutamine, and consume less pyruvate, at the blastocyst compared with cleavage stages. Glucose was consumed by human blastocysts, but not at a hi...Continue Reading

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Citations

Nov 8, 2015·Human Reproduction Update·E BloiseS G Matthews
Feb 20, 2018·Molecular Reproduction and Development·Veronique Duranthon, Pascale Chavatte-Palmer
Dec 23, 2017·Journal of Assisted Reproduction and Genetics·Jason R HerrickRebecca L Krisher
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Aug 11, 2018·Reproduction, Fertility, and Development·Monika NõmmÜlle Jaakma
Dec 11, 2019·Cell Proliferation·Xiao-Wei GuZeng-Ming Yang
Sep 4, 2019·Molecular Reproduction and Development·Caroline G LucasTiago Collares
Oct 1, 2020·International Journal of Molecular Sciences·Ximo Garcia-DominguezFrancisco Marco-Jiménez
May 14, 2021·Biology of Reproduction·Gabriela de Oliveira FernandesMargot Alves Nunes Dode

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Methods Mentioned

BETA
nuclear magnetic resonance
NMR
electrophoresis

Software Mentioned

XCMS
DA
OPLS
PROcess
SIMCA P
R

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