Applying proteomic-based biomarker tools for the accurate diagnosis of pancreatic cancer.

Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract
Kyoko KojimaJohn D Christein

Abstract

The proteome varies with physiologic and disease states. Few studies have been reported that differentiate the proteome of those with pancreatic cancer. To apply proteomic-based technologies to body fluids. To differentiate pancreatic neoplasia from nonneoplastic pancreatic disease. Samples from 50 patients (15 healthy (H), 24 cancer (Ca), 11 chronic pancreatitis (CP)) were prospectively collected and underwent analysis. A high-throughput method, using high-affinity solid lipophilic extraction resins, enriched low molecular weight proteins for extraction with a high-speed 200-Hz matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-MS; Bruker Ultraflex III). Samples underwent software processing with FlexAnalysis, Clinprot, MatLab, and Statistica (baseline, align, and normalize spectra). Nonparametric pairwise statistics, multidimensional scaling, hierarchical analysis, and leave-one-out cross validation completed the analysis. Sensitivity (sn) and specificity (sp) of group comparisons were determined. Two top-down-directed protein identification approaches were combined with MALDI-MS and tandem mass spectrometry to fully characterize the most significant protein biomarker. Using eight serum featur...Continue Reading

References

May 1, 1996·The British Journal of Surgery·C SpertiS Pedrazzoli
Feb 28, 2002·Lancet·Emanuel F PetricoinLance A Liotta
Sep 3, 2005·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·John D ChristeinMichael B Farnell
Nov 29, 2005·Applied Bioinformatics·Milos HauskrechtJames Lyons-Weiler
Feb 22, 2008·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun

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Citations

Feb 15, 2014·Clinica Chimica Acta; International Journal of Clinical Chemistry·Wentao LiuZhenggang Zhu
May 12, 2009·Clinical Chemistry and Laboratory Medicine : CCLM·Filippo NavagliaMario Plebani
Dec 16, 2011·World Journal of Gastroenterology : WJG·Chen SunRoland Andersson
Nov 16, 2011·International Journal of Proteomics·Ruihui GengJun Gao
Feb 13, 2013·International Journal of Proteomics·Alessandra TessitoreEdoardo Alesse
Sep 4, 2015·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Amutha PeriyasamyRajkumar Thangarajan
Mar 20, 2010·Metabolomics : Official Journal of the Metabolomic Society·Masahiro SugimotoMasaru Tomita
Sep 1, 2011·Mass Spectrometry Reviews·Peihong ZhuJohn G Marshall
Oct 7, 2010·Pancreas·Edgar Zapico-MuñizJosefina Mora-Brugués
Jun 27, 2018·EMBO Molecular Medicine·Andrea ResoviRaffaella Giavazzi
Sep 23, 2014·Journal of Cancer Research and Clinical Oncology·Berit VelstraRob A E M Tollenaar

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