Applying the herpes simplex virus thymidine kinase/ganciclovir approach to ovarian cancer: an effective in vitro drug-sensitization system

Gynecologic and Obstetric Investigation
A Al-Hendy, N Auersperg

Abstract

Ovarian cancer is a major clinical problem with no rewarding treatment protocol currently available. In other malignancies transfer of the herpes simplex virus thymidine kinase (HSV-tk) gene into tumor cells using a viral vector followed by administration of ganciclovir (GCV) provides a potentially effective strategy for treatment. In this work human ovarian epithelial cancer cell lines were infected with a recombinant adenoviral vector expressing the HSV-tk (AdRSV-tk) and were rendered sensitive to doses of GCV that were 100-200 times less than for untransfected cells. A strong bystander effect was noted with significant killing at a ratio of infected:uninfected cells of only 1:20 and maximal killing at 1:3. Normal human ovarian surface epithelial cells were also highly sensitive to the AdRSV-tk/GCV system. This study demonstrates the potential efficacy of the HSV-tk/GCV approach in ovarian cancer gene therapy.

Citations

Jan 23, 1998·Journal of Women's Health
Feb 4, 2012·Biology of Reproduction·Sunil K HalderAyman Al-Hendy
May 12, 2000·The Laryngoscope·L L Gleich
Nov 14, 1997·In Vitro Cellular & Developmental Biology. Animal·E H LeungN Auersperg
Aug 3, 2011·Expert Review of Anticancer Therapy·Karli Rosner

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