Appraisal of Biofilm Formation in Diabetic Foot Infections by Comparing Phenotypic Methods With the Ultrastructural Analysis

The Journal of Foot and Ankle Surgery : Official Publication of the American College of Foot and Ankle Surgeons
Sugandhi Pugazhendhi, Arvind Prasanth Dorairaj

Abstract

Diabetic patients are more prone to the development of foot ulcers, because their underlying tissues are exposed to colonization by various pathogenic organisms. Hence, biofilm formation plays a vital role in disease progression by antibiotic resistance to the pathogen found in foot infections. The present study has demonstrated the correlation of biofilm assay with the clinical characteristics of diabetic foot infection. The clinical characteristics such as the ulcer duration, size, nature, and grade were associated with biofilm production. Our results suggest that as the size of the ulcer with poor glycemic control increased, the organism was more likely to be positive for biofilm formation. A high-degree of antibiotic resistance was exhibited by the biofilm-producing gram-positive isolates for erythromycin and gram-negative isolates for cefpodoxime. Comparisons of biofilm production using 3 different conventional methods were performed. The strong producers with the tube adherence method were able to produce biofilm using the cover slip assay method, and the weak producers in tube adherence method had difficulty in producing biofilm using the other 2 methods, indicating that the tube adherence method is the best method for a...Continue Reading

Citations

Sep 20, 2019·The International Journal of Lower Extremity Wounds·Prakhar Srivastava, Karthikeyan Sivashanmugam
Aug 8, 2021·International Journal of Molecular Sciences·Ana C AfonsoManuel Simões

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