Appraisal of the comparative utility of immunohistochemistry and electron microscopy in the diagnosis of childhood round cell tumors

Ultrastructural Pathology
G W MierauD A Weeks

Abstract

To provide an objective assessment of the comparative utility of fluorescence- and peroxidase-based immunohistochemistry and electron microscopy, an observer blinded study was conducted under realistic study conditions utilizing a large sampling of poorly differentiated pediatric round cell tumors. Working independently, using a single ancillary technique of particular expertise, each of three investigators attempted to render a specific diagnosis with regard to 50 diagnostically challenging tumors. The results were compared against the subsequent "file diagnosis" established by consensus with all relevant information made available. A grading scheme was applied wherein points were awarded based on the accuracy and confidence of diagnosis. A comparative efficiency rating, expressed as a percentage, was formulated by dividing the number of points awarded each technique by the total number of points theoretically available. Electron microscopy proved superior overall, with an efficiency rating of 89%. Immunoperoxidase and immunofluorescence studies yielded efficiency ratings of 71 and 61%, respectively. Used in combination, the techniques achieved an efficiency rating of 95%. Application of these ancillary techniques resulted in ...Continue Reading

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Citations

Jul 12, 2002·Oral Oncology·J Hicks, C Flaitz
Dec 29, 1998·Human Pathology·I Dardick, G A Herrera
Nov 30, 2013·Case Reports in Dentistry·Ana Amelia SouzaAndresa Borges Soares
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Feb 24, 2001·Ultrastructural Pathology·J A Tucker
Aug 11, 2000·Applied Immunohistochemistry & Molecular Morphology : AIMM·G A HerreraE A Turbat-Herrera

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