Approach for Downscaling of Electromembrane Extraction as a Lab on-a-Chip Device Followed by Sensitive Red-Green-Blue Detection

Analytical Chemistry
Mahroo BaharfarMuhammad Balal Arain

Abstract

A design of electromembrane extraction (EME) as a lab on-a-chip device was proposed for the extraction and determination of phenazopyridine as the model analyte. The extraction procedure was accomplished by coupling EME and packing a sorbent. The analyte was extracted under the applied electrical field across a membrane sheet impregnated by nitrophenyl octylether (NPOE) into an acceptor phase. It was followed by the absorption of the analyte on strong cation exchanger as a sorbent. The designed chip contained separate spiral channels for donor and acceptor phases featuring embedded platinum electrodes to enhance extraction efficiency. The selected donor and acceptor phases were 0 mM HCl and 100 mM HCl, respectively. The on-chip electromembrane extraction was carried out under the voltage level of 70 V for 50 min. The analysis was carried out by two modes of a simple red-green-blue (RGB) image analysis tool and a conventional HPLC-UV system. After the absorption of the analyte on the solid phase, its color changed and a digital picture of the sorbent was taken for the RGB analysis. The effective parameters on the performance of the chip device, comprising the EME and solid phase microextraction steps, were distinguished and opti...Continue Reading

References

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Citations

Dec 20, 2018·Analytical and Bioanalytical Chemistry·Stig Pedersen-Bjergaard
Apr 23, 2021·Analytical and Bioanalytical Chemistry·Elia SantigosaMaría Ramos-Payán
Mar 5, 2021·Biomedical Chromatography : BMC·Seyed Mosayeb DaryanavardMohamed Abdel-Rehim
May 6, 2019·Analytical Chemistry·Maizatul Najwa JajuliGrégoire Herzog
Sep 26, 2021·Journal of Separation Science·Frederik André HansenStig Pedersen-Bjergaard

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