Approach to Rapid Synthesis and Functionalization of Iron Oxide Nanoparticles for High Gene Transfection

ACS Applied Materials & Interfaces
Zachary R StephenMiqin Zhang

Abstract

Surface functionalization of theranostic nanoparticles (NPs) typically relies on lengthy, aqueous postsynthesis labeling chemistries that have limited ability to fine-tune surface properties and can lead to NP heterogeneity. The need for a rapid, simple synthesis approach that can provide great control over the display of functional moieties on NP surfaces has led to increased use of highly selective bioorthoganol chemistries including metal-affinity coordination. Here we report a simple approach for rapid production of a superparamagnetic iron oxide NPs (SPIONs) with tunable functionality and high reproducibility under aqueous conditions. We utilize the high affinity complex formed between catechol and Fe((III)) as a means to dock well-defined catechol modified polymer modules on the surface of SPIONs during sonochemical coprecipitation synthesis. Polymer modules consisted of chitosan and poly(ethylene glycol) (PEG) copolymer (CP) modified with catechol (CCP), and CCP functionalized with cationic polyethylenimine (CCP-PEI) to facilitate binding and delivery of DNA for gene therapy. This rapid synthesis/functionalization approach provided excellent control over the extent of PEI labeling, improved SPION magnetic resonance imagi...Continue Reading

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Citations

Jun 21, 2017·The Journal of Gene Medicine·M Mar Encabo-BerzosaPilar Martín Duque
Feb 24, 2018·Materials·Lazhen ShenYongsheng Qiao
Sep 25, 2020·Expert Opinion on Drug Delivery·Joydeb Majumder, Tamara Minko
Jan 22, 2019·Royal Society Open Science·Adheesha N DanthanarayanaK M Nalin De Silva
Jul 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Ganeshlenin Kandasamy, Dipak Maity
Jul 22, 2021·Nanoscale Horizons : the Home for Rapid Reports of Exceptional Significance in Nanoscience and Nanotechnolgy·Seokhwan ChungMiqin Zhang
May 29, 2019·Accounts of Chemical Research·Richard A ReviaMiqin Zhang

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