Approaches for In Situ Monitoring of Matrix Development in Hydrogel-Based Engineered Cartilage.

Tissue Engineering. Part C, Methods
Shital KandelNancy Pleshko

Abstract

Near infrared (NIR) spectroscopy using a fiber optic probe shows great promise for the nondestructive in situ monitoring of tissue engineered construct development; however, the NIR evaluation of matrix components in samples with high water content is challenging, as water absorbances overwhelm the spectra. In this study, we established approaches by which NIR spectroscopy can be used to select optimal individual engineered hydrogel constructs based on matrix content and mechanical properties. NIR spectroscopy of dry standard compounds allowed identification of several absorbances related to collagen and/or proteoglycan (PG), of which only two could be identified in spectra obtained from hydrated constructs, at ∼5940 and 5800 cm-1. In dry sample mixtures, the ratio of these peaks correlated positively to collagen and negatively to PG. In NIR spectra from engineered cartilage hydrogels, these peaks reflected higher collagen and PG content and dynamic modulus values, permitting the differentiation of constructs with poor and good matrix development. Similarly, the increasing baseline offset in raw NIR spectra also reflected matrix development in hydrated constructs. However, weekly monitoring of NIR spectra and the peaks at ∼5940...Continue Reading

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Citations

Oct 27, 2020·Cartilage·Jessica M FalconNancy Pleshko
Jan 20, 2021·Nature Protocols·Isaac O AfaraJuha Töyräs
Feb 13, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·William QueridoNancy Pleshko

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Methods Mentioned

BETA
infrared spectroscopy
biopsy
PCA

Software Mentioned

CAMO
Unscrambler X

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