Approaches for Studying Ribosome Specialization

Trends in Biochemical Sciences
Edward EmmottNikolai Slavov

Abstract

Contrary to the textbook model, recent measurements demonstrated unexpected diversity in ribosomal composition that likely enables specialized translational functions. Methods based on liquid chromatography coupled to tandem mass-spectrometry (LC-MS/MS) enable direct quantification of ribosomal proteins with high specificity, accuracy, and throughput. LC-MS/MS can be 'top-down', analyzing intact proteins, or more commonly 'bottom-up', where proteins are digested to peptides prior to analysis. Changes to rRNA can be examined using either LC-MS/MS or sequencing-based approaches. The regulation of protein synthesis by specialized ribosomes can be examined by multiple methods. These include the popular 'Ribo-Seq' method for analyzing ribosome density on a given mRNA, as well as LC-MS/MS approaches incorporating pulse-labelling with stable isotopes (SILAC) to monitor protein synthesis and degradation.

Citations

Aug 21, 2020·Proteomics·Aleksandra A Petelski, Nikolai Slavov
Jul 25, 2019·Cellular and Molecular Life Sciences : CMLS·Shannon E WeeksRajeev S Samant
Jul 17, 2020·Frontiers in Plant Science·Federico Martinez-SeidelJoachim Kopka

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