Approved IFCC reference method for the measurement of HbA1c in human blood

Clinical Chemistry and Laboratory Medicine : CCLM
Jan-Olof JeppssonInternational Federation of Clinical Chemistry and Laboratory Medicine (IFCC)

Abstract

HbA1C is the stable glucose adduct to the N-terminal group of the beta-chain of HbA0. The measurement of HbA1c in human blood is most important for the long-term control of the glycaemic state in diabetic patients. Because there was no internationally agreed reference method the IFCC Working Group on HbA1c Standardization developed a reference method which is here described. In a first step haemoglobin is cleaved into peptides by the enzyme endoproteinase Glu-C, and in a second step the glycated and non-glycated N-terminal hexapeptides of the beta-chain obtained are separated and quantified by HPLC and electrospray ionisation mass spectrometry or in a two-dimensional approach using HPLC and capillary electrophoresis with UV-detection. Both principles give identical results. HbA1c is measured as ratio between the glycated and non-glycated hexapeptides. Calibrators consisting of mixtures of highly purified HbA1c and HbA0 are used. The analytical performance of the reference method has been evaluated by an international network of reference laboratories comprising laboratories from Europe, Japan and the USA. The intercomparison studies of the network showed excellent results with intra-laboratory CVs of 0.5 to 2% and inter-laborat...Continue Reading

References

Sep 30, 1993·The New England Journal of Medicine·UNKNOWN Diabetes Control and Complications Trial Research GroupC Siebert
Jun 15, 1995·Analytical Chemistry·R R Little, D E Goldstein
Jul 24, 1998·Clinical Chemistry and Laboratory Medicine : CCLM·A FinkeJ O Jeppsson
Jun 27, 2000·Diabetes Care·R R Little

❮ Previous
Next ❯

Citations

Aug 23, 2007·Diabetologia·UNKNOWN American Diabetes AssociationUNKNOWN International Diabetes Federation
May 11, 2013·Analytical and Bioanalytical Chemistry·Claudia Swart
Sep 30, 2010·International Journal of Legal Medicine·Cornelius HessBurkhard Madea
May 2, 2008·Annals of Biomedical Engineering·Piotr LadyzyńskiWaldemar Karnafel
Jan 12, 2013·Journal of Pharmacokinetics and Pharmacodynamics·Rocío Lledó-GarcíaMats O Karlsson
Apr 12, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·M Panteghini, J C Forest
Jul 24, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Linda M ThienpontAndré P De Leenheer
Oct 9, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Nathalie SchneiderPhilippe Gillery
Oct 3, 2009·The Journal of Biological Chemistry·Siri MalmgrenVladimir V Sharoyko
Jun 1, 2011·Epidemiologic Reviews·Justin B Echouffo-TcheuguiK M Venkat Narayan
Oct 19, 2010·Journal of Diabetes Investigation·Yutaka SeinoKohjiro Ueki
Mar 30, 2004·Journal of Clinical Pathology·E S Kilpatrick
Dec 19, 2013·BMC Research Notes·Ruziana Mona Wan Mohd ZinWan Nazaimoon Wan Mohamud
Nov 6, 2003·Clinical Chemistry and Laboratory Medicine : CCLM·Randie R Little
Nov 6, 2003·Clinical Chemistry and Laboratory Medicine : CCLM·W Garry John
May 19, 2004·Clinical Chemistry and Laboratory Medicine : CCLM·Sandrine Vallée PolneauSimone Bénazeth
Sep 18, 2007·Clinical Chemistry and Laboratory Medicine : CCLM·Mauro PanteghiniUNKNOWN IFCC Scientific Division
Sep 18, 2007·Clinical Chemistry and Laboratory Medicine : CCLM·UNKNOWN International Federation Of Clinical Chemistry And Laboratory Medicine, IFCC Scientific DivisionRené Dybkaer
Sep 18, 2007·Clinical Chemistry and Laboratory Medicine : CCLM·UNKNOWN International Federation of Clinical Chemistry and Laboratory Medicine, IFCC Scientific DivisionCas W Weykamp
Jul 8, 2008·Clinical Chemistry and Laboratory Medicine : CCLM·Pamela M J McLaughlin, Andries J Bakker
Apr 29, 2009·Clinical Chemistry and Laboratory Medicine : CCLM·Jean SzymezakPhilippe Gillery
Dec 22, 2009·Clinical Chemistry and Laboratory Medicine : CCLM·Jean-Paul ChapelleWouter van den Eshof
Feb 13, 2010·Clinical Chemistry and Laboratory Medicine : CCLM·Yusheng ZhuBeverly D Horne
Mar 6, 2010·Clinical Chemistry and Laboratory Medicine : CCLM·UNKNOWN Glad Working Group A1c Delegates WGMartina Zaninotto
Sep 18, 2010·Clinical Chemistry and Laboratory Medicine : CCLM·Koldo Santos-ReyRaimundo Goberna
Feb 18, 2011·Clinical Chemistry and Laboratory Medicine : CCLM·Cas WeykampWilleke Visser-Dekkers
Feb 18, 2011·Clinical Chemistry and Laboratory Medicine : CCLM·Catalina Sánchez-MoraVíctor Sánchez-Margalet
Mar 18, 2011·Clinical Chemistry and Laboratory Medicine : CCLM·Annunziata LapollaPietro Traldi
May 12, 2007·The Review of Diabetic Studies : RDS·Frantisek SaudekJelena Skibova
Aug 10, 2006·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Yoko NanjoToshio Yao
Oct 30, 2010·Indian Journal of Human Genetics·Jaime J JayapalanSiew P Chan
Oct 12, 2012·International Journal of Medical Sciences·Rolf HinzmannCam Tuan Tran
Jan 16, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Randie R Little, Curt L Rohlfing
Mar 1, 2014·Journal of the American Society for Mass Spectrometry·Shih-Hao WangShu-Hui Chen
Nov 29, 2011·Clinical Chemistry and Laboratory Medicine : CCLM·Catherine O'ConnorFidelma Dunne
Sep 28, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Ali MovahedThomas Netticadan
Oct 10, 2014·Indian Journal of Clinical Biochemistry : IJCB·Bratati SinghSeema Das
Sep 16, 2014·Annales pharmaceutiques françaises·P Gillery
Oct 23, 2012·Analytical and Bioanalytical Chemistry·Luigi LiottaMichaela Luconi
Jan 29, 2014·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Marijn SpeeckaertUNKNOWN European Renal Best Practice Guideline Development Group on Diabetes in Advanced CKD

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Clinical Chemistry and Laboratory Medicine : CCLM
W Garry John
Clinical Chemistry and Laboratory Medicine : CCLM
International Federation of Clinical Chemistry and Laboratory Medicine, IFCC Scientific DivisionCas Weykamp
© 2022 Meta ULC. All rights reserved