Aptamer-based assay for monitoring genetic disorder phenylketonuria (PKU)

International Journal of Biological Macromolecules
Mohammad HasanzadehSoltanali Mahboob

Abstract

The genetic disorder phenylketonuria (PKU) is the inability to metabolize phenylalanine because of a lack of the enzyme phenylalanine hydroxylase. Phenylalanine is used to biochemically form proteins, coded for by DNA. The development of an apta-assay for detection of l-Phenylalanine is presented in this work. A highly specific DNA-aptamer, selected to l-Phenylalanine was immobilized onto a gold nanostructure and electrochemical measurements were performed in a solution containing the phosphate buffer solution with physiological pH. We have constructed an aptamer immobilized gold nanostructure mediated, ultrasensitive electrochemical biosensor (Apt/AuNSs/Au electrode) for l-Phenylalanine detection without any additional signal amplification strategy. The aptamer assemble onto the AuNSs makes Apt/AuNSs/Au electrode an excellent platform for the l-Phenylalanine detection in physiological like condition. Differential pulse voltammetry were used for the quantitative l-Phenylalanine detection. The Apt/AuNSs/Au electrode offers an ultrasensitive and selective detection of l-Phenylalanine down to 0.23 μM level with a wide dynamic range from 0.72 μM-6 mM. The aptasensor exhibited excellent selectivity and stability. The real sample ana...Continue Reading

Citations

Aug 1, 2019·Current Drug Targets·Jobin JoseBijo Mathew
Sep 16, 2020·Biosensors & Bioelectronics·Asiyeh JebelliMiguel de la Guardia
Nov 21, 2018·International Journal of Biological Macromolecules·Arezoo MirzaieAbolghasem Jouyban
Dec 5, 2018·International Journal of Biological Macromolecules·Aziz EftekhariElham Ahmadian
Apr 21, 2019·International Journal of Biological Macromolecules·Ayub KarimzadehNasrin Shadjou
Aug 11, 2019·International Journal of Biological Macromolecules·Ahmad MobedAhad Mokhtarzadeh
Oct 28, 2019·Analytical Chemistry·Erin Marie McConnellYingfu Li
Oct 22, 2019·ACS Sensors·Kevin M CheungAnne M Andrews

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