Aptamer-based competitive electrochemical biosensor for brevetoxin-2

Biosensors & Bioelectronics
Shimaa EissaMohammed Zourob

Abstract

Brevetoxins (BTXs) are very potent marine neurotoxins that increased in geographical distribution in the past decade causing the illness clinically described as neurological shellfish poisoning (NSP). The ethical problems as well as the technical difficulties associated with the currently employed analysis methods for marine toxins are encouraging the research for suitable alternatives to be applied in a regulatory monitoring regime. Here, we report an electrochemical biosensor platform for BTX-2 detection utilising aptamer as specific receptor. Using in vitro selection, high affinity DNA aptamers to BTX-2 were successfully selected for the first time from a large pool of random sequences. The binding of BTX-2 to aptamer pools/clones was monitored using fluorescence and electrochemical impedance spectroscopy (EIS). The aptamer BT10 exhibited the highest binding affinity to BTX-2, with a dissociation constant of 42nM. The effects of the incubation time, pH and metal ions concentrations on the aptamer-toxin binding were studied. The aptamer BT10 was used to construct a label-free competitive impedimetric biosensor for BTX-2 achieving a detection limit of 106pg/ml. We observed a high degree of cross reactivity of the selected apta...Continue Reading

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Citations

Jun 1, 2016·Frontiers in Chemistry·Annamaria Ruscito, Maria C DeRosa
Jul 6, 2016·Frontiers in Chemistry·Franziska Pfeiffer, Günter Mayer
Jun 18, 2016·Analytical and Bioanalytical Chemistry·Morteza Alinezhad NameghiSeyed Mohammad Taghdisi
Oct 18, 2016·Bioconjugate Chemistry·Alejandra TelloHumberto Gomez
Dec 16, 2017·Sensors·Celine I L JustinoTeresa A P Rocha-Santos
Jun 7, 2016·The Analyst·Nuo DuanZhouping Wang
Oct 2, 2015·Chemical Communications : Chem Comm·Yuan ZhaoQijun Song
Jul 18, 2018·Luminescence : the Journal of Biological and Chemical Luminescence·Chongning LiZhiliang Jiang
Jul 25, 2018·Sensors·Isabel CunhaVitor Vasconcelos
Jan 9, 2020·Analytical and Bioanalytical Chemistry·Anabel VillalongaReynaldo Villalonga
Nov 5, 2016·Physiological Measurement·Mojtaba Jafari TadiTero Koivisto
Jan 7, 2017·Journal of Analytical Methods in Chemistry·Rui-Yun TianShi-Ying Lu
Nov 2, 2019·Biosensors·Paloma Yáñez-SedeñoJosé Manuel Pingarrón
May 24, 2019·Biosensors·Yeşeren SaylanAdil Denizli
Feb 9, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cao-An Vu, Wen-Yih Chen
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Apr 13, 2020·Mikrochimica Acta·Farah Asilah AzriSelamat Jinap
Nov 17, 2020·Frontiers in Microbiology·Sushmita PatraJyoti Ranjan Rout
Feb 23, 2021·Emergent Materials·Parsa Pishva, Meral Yüce
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May 1, 2021·Chembiochem : a European Journal of Chemical Biology·Tianjun ChangYingfu Li
Jun 19, 2021·Food Chemistry·Monali MukherjeePraveena Bhatt
Jul 4, 2021·Talanta·Saipriya RamalingamAshutosh Singh
Jul 18, 2018·Environmental Science & Technology·Spurti U Akki, Charles J Werth
Jan 21, 2017·Analytical Chemistry·Yan Du, Shaojun Dong
Mar 8, 2017·Analytical Chemistry·Shimaa Eissa, Mohammed Zourob

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