Aptamer based electrochemical assay for the determination of thrombin by using the amplification of the nanoparticles

Biosensors & Bioelectronics
Caifeng DingJin-Ming Lin

Abstract

A novel electrochemical assay based on the aptamer and the signal of amplification of nanoparticles (NPs) was constructed for the determination of thrombin. Aptamers immobilized on the electrode and Au NPs could be assembled with the target protein to form a sandwich structure in the presence of the latter. Differential pulse voltammetry (DPV) was employed to detect the CdS NPs loaded on the surface of the Au NPs through the linker DNA, which was related to the concentration of the target protein. The assay took advantage of the amplification ability of Au nanoparticles carrying multiplex CdS NPs and the specific affinity of aptamers. Thrombin was detected in this assay in the linear range of 1.0x10(-15) to 1.0x10(-11) M with the detection limit of 5.5x10(-16) M of target protein. In addition, the assay could be used to detection thrombin in real samples with high sensitivity and good selectivity.

References

Jan 17, 1995·Biochemistry·D E Huizenga, J W Szostak

❮ Previous
Next ❯

Citations

Feb 22, 2012·Analytical and Bioanalytical Chemistry·Ilaria Palchetti, Marco Mascini
Aug 9, 2011·Accounts of Chemical Research·Michael Famulok, Günter Mayer
Dec 14, 2011·Sensors·Sheng-Fu LinJenq-Yang Chang
Aug 5, 2014·Bioelectrochemistry·Maryam MohamadiMasoud Torkzadeh-Mahani
Jul 21, 2010·Analytical and Bioanalytical Chemistry·Jing ZhouYong Wang
May 11, 2013·Chemical Communications : Chem Comm·Mar OrovalPedro Amorós
Jan 14, 2017·Australasian Physical & Engineering Sciences in Medicine·Tao-Sheng HuangYun-Shao He
Jan 4, 2012·Angewandte Chemie·Marco MasciniSara Tombelli
Aug 24, 2010·Biosensors & Bioelectronics·Pinar KaraArben Merkoçi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.