Aptamer-based electrochemical sensors that are not based on the target binding-induced conformational change of aptamers

The Analyst
Ying LuLanqun Mao

Abstract

This study describes a new kind of aptamer-based electrochemical sensor that is not based on the target binding-induced conformational change of the aptamers by using a 15-mer thrombin-binding aptamer (5'-GGTTGGTGTGGTTGG-3') as the model oligonucleotide. The sensors are developed by first self-assembling the aptamer (i.e. a thrombin-binding aptamer) onto an Au electrode and then hybridizing the assembled aptamer with a ferrocene (Fc)-labeled short aptamer-complementary DNA oligonucleotide to form an electroactive double-stranded DNA (ds-DNA) oligonucleotide onto the Au electrode. The binding of the target (i.e. thrombin) towards the aptamer essentially destroys the Watson-Crick helix structure of the ds-DNA oligonucleotide assembled onto the electrode and leads to the dissociation of the Fc-labeled short complementary DNA oligonucleotide from the electrode surface to the solution, resulting in a decrease in the current signal obtained at the electrode, which can be used for the determination of the target. With the thrombin-binding aptamer as the model oligonucleotide, the current decrease obtained with the aptamer-based electrochemical sensors is linear with the concentration of thrombin within the concentration range from 0 t...Continue Reading

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Citations

Mar 6, 2012·Biosensors & Bioelectronics·Jing ZhaoGenxi Li
Feb 14, 2012·Biosensors & Bioelectronics·Marimuthu CitartanThean-Hock Tang
Jul 4, 2015·Biosensors & Bioelectronics·Onur Parlak, Anthony P F Turner
Feb 7, 2012·Biosensors & Bioelectronics·Lingsong LuGuoming Xie
Mar 18, 2011·Faraday Discussions·Anthony E G Cass, Yangyang Zhang
Oct 31, 2013·Chemical Society Reviews·Yiting TangHua-Zhong Yu
Jan 15, 2021·Diagnostics·Ragaa Abd-Ellatief, Maha Ragaa Abd-Ellatief
Feb 4, 2010·Analytical Chemistry·Nazgul TuleuovaAlexander Revzin
Apr 10, 2020·Analytical Chemistry·Liangliang ZhangFengyu Li

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