Aptamer-Based Field-Effect Transistor for Detection of Avian Influenza Virus in Chicken Serum.

Analytical Chemistry
Jae KwonJae-Hyuk Ahn

Abstract

Early diagnosis of the highly pathogenic H5N1 avian influenza virus (AIV) is significant for preventing and controlling a global pandemic. However, there is no existing electrical biosensor for detecting biomarkers for AIV in clinically relevant samples such as chicken serum. Herein, we report the first use of an aptamer-functionalized field-effect transistor (FET) as a label-free sensor for AIV detection in chicken serum. A DNA aptamer is employed as a sensitive and selective receptor for hemagglutinin (HA) protein, which is a biomarker for AIVs. This aptamer is immobilized on a gold microelectrode that is connected to the gate of a reusable FET transducer. The specific binding of the target protein results in a change in the surface potential, which generates a signal response of the FET transducer. We hypothesize that a conformational change in the DNA aptamer upon specific binding of HA protein may alter the surface potential. The signal of the aptamer-based FET biosensor increased linearly with the increase in the logarithm of HA protein concentration in a dynamic range of 10 pM to 10 nM with a detection limit of 5.9 pM. The selectivity of the biosensor for HA protein was confirmed by employing relevant interfering protein...Continue Reading

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Citations

Dec 18, 2020·Frontiers in Plant Science·Arshak PoghossianMichael J Schöning
Feb 6, 2021·Sensors·Samy M Shaban, Dong-Hwan Kim
Apr 4, 2021·Sensors·Ji Hyun KimJae-Hyuk Ahn
Apr 13, 2021·Frontiers of Environmental Science & Engineering·Jinbiao MaQian Zhang
May 1, 2021·International Journal of Molecular Sciences·Tae-Hyeong Kim, Seong-Wook Lee
May 1, 2021·Biosensors·Abbas PanahiEbrahim Ghafar-Zadeh
Apr 15, 2021·Chemistry, an Asian Journal·Cong HanLiyun Zhang
Jun 5, 2021·Applied Biochemistry and Biotechnology·Hongli FanZhuoya Luo
Jul 10, 2021·World Journal of Microbiology & Biotechnology·Tholasi Nadhan NavienMarimuthu Citartan
Nov 21, 2020·Analytical Chemistry·Mohamed Sharafeldin, Jason J Davis
Oct 9, 2021·Journal of the American Chemical Society·Derong KongDacheng Wei

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