Aptamer based high throughput colorimetric biosensor for detection of staphylococcus aureus.

Scientific Reports
Tianxiao YuJun Ge

Abstract

To develop a high throughput colorimetric biosensor for detection of Staphylococcus aureus (SA) based on specific aptamer and catalysis of dsDNA-SYBR Green I (SG I) complex. SA specific aptamer was immobilized on a 96-well plate by hybridization with the capture probe anchored on the plate surface through streptavidin-biotin binding. In presence of SA, the aptamer was dissociated from the capture probe-aptamer duplex due to the stronger interaction between the aptamer and SA. The consequent single-strand capture probe could be hybridized with a three-way junction (TWJ) probe. With the presence of SG I, the dsDNA-SG I complex catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) under photo-irradiation, producing sensitive photo-catalyzed colorimetric response to SA. Under the optimal conditions, the proposed method could directly detect SA with the limit of detection (LOD) at 81 CFU mL-1 in PBS buffer in 5.5 hours, which demonstrated the sensitive and fast quantification of target pathogenic bacteria. The method showed weak colorimetric signal to Escherichia coli and Pseudomonas aeruginosa, indicating the high specificity for SA. In addition, the method can simultaneously detect 96 samples which can be used for high th...Continue Reading

References

Jan 16, 2003·Bioconjugate Chemistry·Lasse VälimaaTimo Lövgren
Jan 17, 2012·Accounts of Chemical Research·Chao WangXi Zhang
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Sep 28, 2017·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·S RoisinO Denis

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Citations

Jul 28, 2020·International Journal of Molecular Sciences·Nevina E Trunzo, Ka Lok Hong
Oct 11, 2020·International Journal of Molecular Sciences·Chioma Izzi-EngbeayaWaljit S Dhillo
Nov 24, 2020·Biochip Journal·Soo Min KimTaek Lee
Mar 19, 2021·ACS Infectious Diseases·Carly DeusenberyAnita Shukla
May 1, 2021·Nanomaterials·Olubunmi O AyodeleTetyana Ignatova
Jun 25, 2021·Advanced Healthcare Materials·Kaiyi ZhangXiaodong Chen

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
PCR
biosensor
biosensing
ELISA

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